Circulating Prostate Cells Found in Men with Benign Prostate Disease Are P504S Negative: Clinical Implications.
Circulating Prostate Cells Found in Men with Benign Prostate Disease Are P504S Negative: Clinical Implications.
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DOI：
10.1155/2013/165014
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发表时间：
2013
影响因子：
--
通讯作者：
Dueñas R
中科院分区：
文献类型：
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作者：
Murray NP;Reyes E;Badínez L;Orellana N;Fuentealba C;Olivares R;Porcell J;Dueñas R
导论.基于免疫学和定量实时PCR的分析的发展使得能够检测、计数和表征循环肿瘤细胞（CTC）。假设CTC的检测与癌症相关，这是基于CTC可以在所有主要癌症中检测到而在健康受试者或患有良性疾病的受试者中检测不到的发现。方法和病人。疑似前列腺癌的患者在前列腺活检前采集血液样本;采用差速凝胶离心法获得单核细胞，并采用抗PSA免疫细胞化学法检测CPC。阳性样品用抗P504 S进行进一步分类。结果329名男性接受了前列腺活检;其中83名男性接受了第二次活检，44名男性接受了第三次活检。在癌症活检阴性的患者中，19/226（8.4%）在第一次活检时检测到CPC PSA（+）P504 S（−），6/74（8.1%）在第二次活检时检测到CPC PSA（+）P504 S（−），5/33（15.2%）在第三次活检时检测到CPC PSA（+）P504 S（−）。癌症活检阳性的男性没有检测到PSA（+）P504 S（−）CPC。这些良性细胞与慢性前列腺炎有关。结论.慢性前列腺炎患者可能在血液中检测到循环前列腺细胞，这些细胞不表达P504 S酶，应该被认为是良性的。
Introduction. Developments in immunological and quantitative real-time PCR-based analysis have enabled the detection, enumeration, and characterization of circulating tumor cells (CTCs). It is assumed that the detection of CTCs is associated with cancer, based on the finding that CTCs can be detected in all major cancer and not in healthy subjects or those with benign disease. Methods and Patients. Consecutive men, with suspicion of prostate cancer, had blood samples taken before prostate biopsy; mononuclear cells were obtained using differential gel centrifugation and CPCs detecting using anti-PSA immunocytochemistry. Positive samples underwent further classification with anti-P504S. Results. 329 men underwent prostate biopsy; of these men 83 underwent a second biopsy and 44 a third one. Of those with a biopsy negative for cancer, 19/226 (8.4%) had CPCs PSA (+) P504S (−) detected at first biopsy, 6/74 (8.1%) at second biopsy, and 5/33 (15.2%) at third biopsy. Men with cancer-positive biopsies did not have PSA (+) P504S (−) CPCs detected. These benign cells were associated with chronic prostatitis. Conclusions. Patients with chronic prostatitis may have circulating prostate cells detected in blood, which do not express the enzyme P504S and should be thought of as benign in nature.