Neisseria lactamica Controlled Human Infection Model.
Neisseria lactamica Controlled Human Infection Model.
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DOI：
10.1007/978-1-0716-1900-1_21
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发表时间：
2022-01-01
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影响因子：
--
通讯作者：
Laver, Jay R
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文献类型：
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作者：
Dale, Adam P;Gbesemete, Diane F;Laver, Jay R
内酰胺奈瑟菌是人类上呼吸道的一种非致病性病原体，与抗N.脑膜炎的定植和疾病。我们以前使用N。采用人感染模型，观察N. N.脑膜炎球菌定植，交叉反应性免疫反应的性质安装到N。脑膜炎继N. lactamica定殖和N.在5个月的定殖期内，最近，我们已经评估了利用转基因N.内酰胺，以使得能够使用所述唾液酸作为媒介物，用于使人鼻咽长期暴露于在携带的生物体中表达的感兴趣的抗原。控制感染N.已经实施了表达脑膜炎球菌抗原NadA的内酰胺，并且结果证明该策略在产生针对靶抗原的免疫应答方面是有效的。在本章中，我们将逐步概述在进行受控的人类感染N.包括与以下有关的程序：（1）稀释N. lactamica储备小瓶以获得鼻内接种物，（2）将鼻内接种物递送至人类志愿者，（3）测定N.采用口咽拭子和鼻腔冲洗液采样法测定鼻内接种后的内酰胺菌定植状况;研究志愿者中的内酰胺定植;（5）鉴定N.用聚合酶链反应鉴定菌株Y 92 -1009的菌落。
Neisseria lactamica is a nonpathogenic commensal of the human upper respiratory tract that has been associated with protection against N. meningitidis colonization and disease. We have previously utilized the N. lactamica controlled human infection model to investigate the protective effect of N. lactamica colonization on N. meningitidis colonization, the nature of cross-reactive immune responses mounted toward N. meningitidis following N. lactamica colonization, and the microevolution of N. lactamica over a 5-month colonization period. More recently, we have assessed the possibility of utilizing genetically modified strains of N. lactamica to enable use of the commensal as a vehicle for prolonged exposure of the nasopharynx of humans to antigens of interest, expressed in carried organisms. A controlled infection with N. lactamica expressing the meningococcal antigen NadA has been executed and the results demonstrate that this strategy is effective at generating immune responses to the target antigen. Throughout this chapter, we outline in a step-by-step manner the methodologies utilized when performing controlled human infection with N. lactamica including procedures relating to: (1) the dilution of N. lactamica stock vials to derive intranasal inocula, (2) the delivery of intranasal inocula to human volunteers, (3) the determination of N. lactamica colonization status following intranasal inoculation using oropharyngeal swabbing and nasal wash sampling, (4) the microbiological procedures utilized to identify N. lactamica colonization among study volunteers, and (5) the identification of N. lactamica colonies as strain Y92-1009 using polymerase chain reaction.