Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium
Statistical media optimization for growth and PHB production from methanol by a methylotrophic bacterium
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DOI：
10.1016/j.biortech.2008.11.024
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发表时间：
2009-04-01
影响因子：
11.4
通讯作者：
Darani, Kianoush Khosravi
中科院分区：
文献类型：
--
作者：
Mokhtari-Hosseini, Zahra B.;Vasheghani-Farahani, Ebrahim;Darani, Kianoush Khosravi
通过统计设计优化了该菌DSMZ 1340的细胞生长和PHB产量的培养条件。采用中心组合设计对生长培养基中的四个重要成分进行了优化。生长速度从对照培养的OD=1.35提高到优化培养的OD=2.15。然后对PHB生产的培养基组分进行了优化。用响应面方法对五个重要因素进行了优化。结果表明，PHB产生菌的最适培养基组成为7.8g/L的Na2HPO4中心点12H(2)O，而(NH_4)(2)SO_4、KH_2PO_4、MgSO_4和MnSO_4的最佳浓度为零。在此条件下，PHB的产量为2.95g/L。RSM结果表明，氮和镁的缺乏是PHB在该微生物中积累的关键。在优化的发酵条件下，在5号L发酵罐中进行了PHB的生产，得到了9.5g/L的PHB和15.4g/L的细胞干重，聚合体含量为62.3%。(C)2008年，爱思唯尔有限公司出版。
Media components were optimized by statistical design for cell growth and PHB production of Methylobacterium extorquens DSMZ 1340. Four important components of growth media were optimized by central composite design. The growth increased from an OD = 1.35 for Choi medium as control to an OD = 2.15 for optimal medium. Then media components for PHB production were optimized. Optimization of five important factors was conducted by response surface method. The optimal composition of PHB production medium was found to be at 7.8 (g/L) Na2HPO4 center dot 12H(2)O, and surprisingly at zero concentration of (NH4)(2)SO4, KH2PO4, MgSO4 and MnSO4. The PHB production was found to be 2.95 (g/L) at this medium. RSM results indicated that a deficiency of nitrogen and magnesium is crucial for PHB accumulation in this microorganism. Also, PHB production was carried out in a 5 L fermentor at the optimum condition which resulted in 9.5 g/L PHB and 15.4 g/L cell dry weight with 62.3% polymer content. (c) 2008 Published by Elsevier Ltd.