Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell‐induced by angiotensin II
Inhibitory effects of emodin on the proliferation of cultured rat vascular smooth muscle cell‐induced by angiotensin II
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DOI：
10.1002/ptr.2301
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发表时间：
2008-02
影响因子：
7.2
通讯作者：
Shi-Jun Wang;Yun-ying Liu;Fang-hua Fan;Jie Yan;Xingxiang Wang;Jun-zhu Chen
中科院分区：
文献类型：
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作者：
Shi-Jun Wang;Yun-ying Liu;Fang-hua Fan;Jie Yan;Xingxiang Wang;Jun-zhu Chen
人参作为一种传统中药使用了几个世纪，为心血管和其他疾病提供了治疗潜力。大黄素，从大黄的根提取物中提取，引起了越来越多的治疗应用的兴趣。本研究旨在探讨大黄素对血管紧张素II（Ang II）诱导的血管平滑肌细胞（VSMCs）增殖及原癌基因c-myc表达的影响。通过外植体法培养VSMC，然后与大黄素（10-80 μm）和Ang II孵育24、48和72 h，或不处理（对照）。MTT法检测细胞增殖活性;免疫组化法检测增殖细胞核抗原（PCNA）表达。采用免疫组织化学染色和图像分析技术检测c-myc的表达。与对照组相比，Ang Ⅱ可促进细胞增殖（p < 0.01）。PCNA和c-myc表达较对照组明显增强（p < 0.01）。大黄素预处理后上述指标较Ang Ⅱ组明显降低（p < 0.01）。大黄素对Ang Ⅱ诱导的VSMCs增殖有抑制作用。c-myc表达的抑制可能与抑制作用相关。版权所有© 2007约翰威利父子有限公司。
Rhubarb, used as a traditional Chinese medicine for centuries, offers therapeutic potential for cardiovascular and other diseases. Emodin, extracted from the root extract of rhubarb has sparked increasing interest for therapeutic application. The main objective was to study the effect of emodin on cultured vascular smooth muscle cells (VSMCs) proliferation induced by angiotensin II (Ang II) and the expression of proto‐oncogene c‐myc. VSMCs were cultured by the explant method, then incubated for 24, 48 and 72 h with emodin (10–80 µm) and Ang II, or were left untreated (control). Cell proliferation was measured by MTT assay and immunohistochemical staining for proliferating cell nuclear antigen (PCNA), respectively. The expression of c‐myc was measured by immunohistochemical staining and image analysis technique. Ang II increased the cell proliferation compared with the control group (p < 0.01). The expression of PCNA and c‐myc was increased compared with the control group (p < 0.01). After pretreatment with emodin, the above indexes were obviously reduced compared with the Ang II group (p < 0.01). These findings suggested that emodin inhibited VSMCs proliferation induced by Ang II. Inhibition of the expression of c‐myc might be correlated with the inhibitory effects. Copyright © 2007 John Wiley & Sons, Ltd.