Virulence Gene Profiles and Population Genetic Analysis for Exploration of Pathogenic Serogroups of Shiga Toxin-Producing Escherichia coli
Virulence Gene Profiles and Population Genetic Analysis for Exploration of Pathogenic Serogroups of Shiga Toxin-Producing Escherichia coli
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DOI：
10.1128/jcm.01598-13
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发表时间：
2013-09
影响因子：
9.4
通讯作者：
Naoki Kobayashi;Ken-ichi Lee;A. Yamazaki;S. Saito;I. Furukawa;Tomomi Kono;E. Maeda;J. Isobe;Y. Sugita‐Konishi;Y. Hara-Kudo
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文献类型：
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作者：
Naoki Kobayashi;Ken-ichi Lee;A. Yamazaki;S. Saito;I. Furukawa;Tomomi Kono;E. Maeda;J. Isobe;Y. Sugita‐Konishi;Y. Hara-Kudo
产滋贺毒素（Stx）大肠杆菌（STEC）感染是一个严重的公共卫生问题，可引起严重腹泻和溶血性尿毒症综合征。STEC菌株之间的患者症状是不同的，可能意味着除了Stx之外的STEC毒力标志物的存在。为了揭示导致STEC毒力的基因型特征，我们研究了不同血清群的282株菌株中17个主要毒力基因的存在，即，stx 1、stx 2a、stx 2c、stx 2d、stx 2e、stx 2f、eae、tir、espB、espD、iha、saa、subA、ehxA、espP、katP和stcE。接下来，我们根据血清病理型检查了毒力基因的流行，其中血清型根据人类疾病中报告的频率以及与暴发和严重疾病的已知关联进行了分类（血清病理型A至E）。我们的研究结果表明，除了位于肠上皮细胞消失（LEE）位点的基因（包括eae、tir、espB和espD）外，STEC中katP和stcE的存在可能指示STEC的最致病基因型。毒力基因谱的人口结构分析在统计上支持致病基因型，而且，发现有潜在的致病性比以前认为的更高的血清群。血清群O26、O145和O165中的一些菌株可能具有与血清群O157相当的高毒力。包括O14、O16、O45、O63、O74、119、O128和O无法分型的几个血清群也可能是潜在致病性的，尽管在人类中很少。
ABSTRACT Infection with Shiga toxin (Stx)-producing Escherichia coli (STEC) is a serious public health concern, causing severe diarrhea and hemolytic-uremic syndrome. Patient symptoms are varied among STEC strains, possibly implying the presence of markers for STEC virulence other than Stx. To reveal the genotypic traits responsible for STEC virulence, we investigated 282 strains of various serogroups for the presence of 17 major virulence genes, i.e., stx 1, stx 2a, stx 2c, stx 2d, stx 2e, stx 2f, eae, tir, espB, espD, iha, saa, subA, ehxA, espP, katP, and stcE. Next, we examined the prevalence of virulence genes according to the seropathotypes in which serotypes were classified (seropathotypes A through E) based on the reported frequencies in human illness, as well as known associations with outbreaks and with severe disease. Our results demonstrate that the presence of both katP and stcE in STEC, in addition to the genes located in the locus of enterocyte effacement (LEE), including eae, tir, espB, and espD, may indicate the most pathogenic genotype of STEC. A population structure analysis of the profiles of virulence genes statistically supported the pathogenic genotype and, furthermore, revealed that there are serogroups with potentially higher pathogenicity than previously thought. Some strains in serogroups O26, O145, and O165 may have high virulence equivalent to that of serogroup O157. Several serogroups, including O14, O16, O45, O63, O74, 119, O128, and O untypeable, also may be potentially pathogenic, although rarely in humans.