Adipose-Derived Mesenchymal Stem Cells in Collagen Hyaluronic Acid Gel Composite Scaffolds for Vocal Fold Regeneration
Adipose-Derived Mesenchymal Stem Cells in Collagen Hyaluronic Acid Gel Composite Scaffolds for Vocal Fold Regeneration
复制标题
胶原透明质酸凝胶复合支架中的脂肪源间充质干细胞用于声带再生
DOI：
10.1177/000348941112000209
复制
发表时间：
2011-02-01
影响因子：
1.4
通讯作者：
Han, Demin
中科院分区：
文献类型：
--
作者：
Xu, Wen;Hu, Rong;Han, Demin
目的：研究兔声带创伤模型中自体脂肪间充质干细胞(ADSCs)植入复合支架后固有层细胞外基质(ECM)的变化。方法：ADSCs与胶原或透明质酸凝胶共同培养。声带局部切除损伤，注射ADSC复合体、ADSCs或支架，或不治疗。结果：植入ADSC复合体后，3个月时声带胶原含量明显增加，分布紊乱。随后开始下降，到12个月时接近正常。透明质酸的含量在40d时增加，但在接下来的12个月内下降到正常水平，仅限于固有层的浅层和中层。纤维连接蛋白在固有层中继续分散，在40天达到高峰，然后在接下来的12个月中下降到接近正常水平。结论：ADSC复合体在声带再生中起促进作用，调节细胞外基质的生成和有序分布。
Objectives: We sought to characterize the changes in the extracellular matrix (ECM) of the lamina propria following the implantation of autologous adipose-derived mesenchymal stem cells (ADSCs) in composite scaffolds in a rabbit vocal fold wound model.Methods: The ADSCs were co-cultured with collagen or hyaluronic acid gel. Each vocal fold was injured by localized resection and injected with ADSC complexes, ADSCs, or scaffolds or left untreated. From 15 days to 12 months, the vocal fold shape and the characteristics of the ECM components were analyzed.Results: With implantation of the ADSC complexes, the collagen content was significantly increased and had a disorderly distribution at 3 months. Subsequently, it began to decrease and reached close to normal by 12 months. The hyaluronic acid content was increased at 40 days, but it was reduced to normal levels and was limited to the superficial and middle layers of the lamina propria over the following 12 months. Fibronectin continued to be scattered in the lamina propria, at peak levels at 40 days, and then decreased over the following 12 months to reach near-normal levels. At 12 months, the vocal folds had a normal surface.Conclusions: ADSC complexes can play a facilitatory role in vocal fold regeneration, regulating the generation and orderly distribution of ECM.