Osthole inhibits proliferation of human breast cancer cells by inducing cell cycle arrest and apoptosis.
Osthole inhibits proliferation of human breast cancer cells by inducing cell cycle arrest and apoptosis.
复制标题
蛇床子素通过诱导细胞周期停滞和细胞凋亡来抑制人乳腺癌细胞的增殖
DOI：
10.7555/jbr.27.20120115
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发表时间：
2015-04
影响因子：
2.3
通讯作者：
Ma C
中科院分区：
文献类型：
--
作者：
Wang L;Peng Y;Shi K;Wang H;Lu J;Li Y;Ma C
摘要蛇床子素是从蛇床子中分离得到的有效成分。苦参是一种具有抗癌活性的中药。然而，其对乳腺癌细胞的作用迄今尚未阐明清楚。本研究探讨蛇床子素对人乳腺癌细胞MDA-MB 435增殖、细胞周期和凋亡的影响。蛇床子素能有效抑制MDA-MB 435细胞的增殖，其诱导的细胞凋亡是通过caspase-9和caspase-3的激活和PARP的降解来实现的。其诱导G1期阻滞的机制可能与上调p53和p21的表达，下调Cdk 2和cyclin D1的表达有关。综上所述，蛇床子素的抗肿瘤作用可能是通过诱导乳腺癌细胞周期阻滞和凋亡来实现的，蛇床子素可能是一种潜在的抗乳腺癌化疗药物。
Abstract Recent studies have revealed that osthole, an active constituent isolated from the fruit of Cnidium monnieri (L.) Cusson, a traditional Chinese medicine, possesses anticancer activity. However, its effect on breast cancer cells so far has not been elucidated clearly. In the present study, we evaluated the effects of osthole on the proliferation, cell cycle and apoptosis of human breast cancer cells MDA-MB 435. We demonstrated that osthole is effective in inhibiting the proliferation of MDA-MB 435 cells, The mitochondrion-mediated apoptotic pathway was involved in apoptosis induced by osthole, as indicated by activation of caspase-9 and caspase-3 followed by PARP degradation. The mechanism underlying its effect on the induction of G1 phase arrest was due to the up-regulation of p53 and p21 and down-regulation of Cdk2 and cyclin D1 expression. Were observed taken together, these findings suggest that the anticancer efficacy of osthole is mediated via induction of cell cycle arrest and apoptosis in human breast cancer cells and osthole may be a potential chemotherapeutic agent against human breast cancer.