Performing discovery-driven neonatal research by transcriptomic analysis of routinely discarded biofluids.
Performing discovery-driven neonatal research by transcriptomic analysis of routinely discarded biofluids.
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DOI：
10.3109/14767058.2012.717126
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
Bianchi DW
中科院分区：
文献类型：
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作者：
Maron JL;Dietz JA;Parkin C;Johnson KL;Bianchi DW
对新生儿唾液和脐带血转录本进行发现驱动的研究。两组不同的婴儿参加了这项研究。其中一组在足月妊娠时采集脐带血(n=10)，另一组(n=10)采集唾液样本。提取总RNA，扩增后与Affymetrix HG U133a基因表达芯片杂交。归一化后，在每种生物体液中所有受试者中表达最高五分之一(≥80%)的基因用独创性路径分析进行分析。探索了与新生儿器官特定发育和生理功能相关的过度表达的途径。符合统计标准的新生儿唾液基因有303个，脐带血基因有282个。其中，114种是两种生物体液共同的。通路分析揭示了氧化还原平衡、细胞增殖和平滑肌松弛的重要作用。在血液中，造血和免疫反应途径占主导地位。在唾液中，与胃肠道系统相关的通路被突出显示。新生儿脐带血和唾液提供了丰富的转录信息。这些通常被丢弃的生物液应该被认为是实时基因表达数据的重要来源，这些数据可能会阐明新生儿生理和病理中的关键途径。
To perform discovery-driven research on the neonatal salivary and cord blood transcriptomes. Two separate cohorts of infants were enrolled in this study. In one, cord blood (n = 10) and in the other, saliva samples (n = 10) were collected at term gestation. Total RNA was extracted, amplified and hybridized onto Affymetrix HG U133a gene expression microarrays. Following normalization, genes expressed in the highest quintile (≥ 80%) across all subjects in each biofluid were analyzed with Ingenuity Pathway Analysis. Over-represented pathways relating to organ specific development and physiological functions in the newborn were explored. There were 303 genes in neonatal saliva and 282 genes in umbilical cord blood that met statistical criteria. Of these, 114 were common to both biofluids. Pathway analyses revealed the important roles of redox balance, cellular proliferation, and smooth muscle relaxation. In blood, hematopoiesis and immune response pathways predominated. In saliva, pathways associated with the gastrointestinal system were highlighted. Neonatal cord blood and saliva provide a wealth of transcriptomic information. These normally discarded biofluids should be considered an important source of real-time gene expression data that may elucidate key pathways in neonatal physiology and pathology.