Expression of Activating Transcription Factor 3 (ATF 3) and caspase 3 in Schwann cells and axonal outgrowth after sciatic nerve repair in diabetic BB rats
Expression of Activating Transcription Factor 3 (ATF 3) and caspase 3 in Schwann cells and axonal outgrowth after sciatic nerve repair in diabetic BB rats
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DOI：
10.1016/j.neulet.2012.03.011
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发表时间：
2012-04-25
影响因子：
2.5
通讯作者：
Dahlin, Lars B.
中科院分区：
文献类型：
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作者：
Stenberg, Lena;Kanje, Martin;Dahlin, Lars B.
本研究的目的是评价糖尿病BB大鼠（即显示1型糖尿病表型）雪旺细胞中与转录因子激活转录因子3（ATF 3）和凋亡标志物半胱天冬酶3相关的神经再生。将健康Wistar大鼠和糖尿病BB大鼠的坐骨神经横断并立即修复。六天后通过免疫组织化学定量轴突生长（神经丝染色）和ATF 3和caspase 3的表达。健康和糖尿病大鼠之间的轴突生长没有差异。然而，在糖尿病大鼠的坐骨神经中，在病变部位表现出更多数量的ATF 3表达的雪旺细胞，并且还表现出更多数量的半胱天冬酶3表达的雪旺细胞。糖尿病大鼠和健康大鼠的远端神经节段也存在类似的差异。表达ATF 3和caspase 3的雪旺细胞数之间无相关性。因此，糖尿病BB大鼠显示ATF 3的活化增加，并且表达许旺细胞的凋亡半胱天冬酶3也增加，但在神经损伤和修复后6天的轴突生长长度没有差异。了解糖尿病应激后的信号转导机制可能为糖尿病神经病变和神经病理性疼痛的发展提供新的见解。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
The aim of this study was to evaluate nerve regeneration in relation to the transcription factor, Activating Transcription Factor 3 (ATF 3), and an apoptotic marker, caspase 3, in the Schwann cells of diabetic BB rats (i.e. display type 1 diabetes phenotype). Sciatic nerves in healthy Wistar rats and in diabetic BB rats were transected and immediately repaired. Axonal outgrowth (neurofilament staining) and expression of ATF 3 and caspase 3 were quantified by immunohistochemistry after six days. There was no difference in axonal outgrowth between healthy and diabetic rats. However, the sciatic nerve in the diabetic rats exhibited a larger number of ATF 3 expressing Schwann cells at the site of the lesion and also a higher number of caspase 3 expressing Schwann cells. Similar differences were observed in the distal nerve segment between the healthy and diabetic rats. There were no correlations between the number of Schwann cells expressing ATF 3 and caspase 3. Thus, diabetic BB rats display an increased activation of ATF 3 and also a rise in apoptotic caspase 3 expressing Schwann cells, but with no discrepancy in length of axonal outgrowth after nerve injury and repair at six days. Knowledge about signal transduction mechanisms in diabetes after stress may provide new insights into the development of diabetic neuropathy and neuropathic pain. (C) 2012 Elsevier Ireland Ltd. All rights reserved.