Disruption and eradication of P. aeruginosa biofilms using nitric oxide-releasing chitosan oligosaccharides.
Disruption and eradication of P. aeruginosa biofilms using nitric oxide-releasing chitosan oligosaccharides.
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DOI：
10.1080/08927014.2015.1107548
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发表时间：
2015
期刊：
影响因子：
2.7
通讯作者：
Schoenfisch MH
中科院分区：
文献类型：
--
作者：
Reighard KP;Hill DB;Dixon GA;Worley BV;Schoenfisch MH
研究了生物膜的破坏和清除情况，将其作为释放一氧化氮（NO）的壳寡糖剂量的函数，并将结果与对照（即不释放NO的）壳寡糖和妥布霉素进行比较。使用生物膜扩张/收缩的定量分析和多粒子追踪微观流变学来评估抗菌处理前后生物膜的结构完整性。妥布霉素对生物膜的物理性质没有影响，而释放NO的壳寡糖在生物膜降解方面表现出剂量依赖性。对照壳寡糖增加了生物膜的弹性，这表明该支架可能在一定程度上减轻了一氧化氮对生物膜的破坏能力。这项研究的结果表明，释放一氧化氮的壳寡糖可作为双重作用的治疗剂，能够清除和物理破坏铜绿假单胞菌生物膜。
Biofilm disruption and eradication were investigated as a function of nitric oxide- (NO) releasing chitosan oligosaccharide dose with results compared to control (ie non-NO-releasing) chitosan oligosaccharides and tobramycin. Quantification of biofilm expansion/contraction and multiple-particle tracking microrheology were used to assess the structural integrity of the biofilm before and after antibacterial treatment. While tobramycin had no effect on the physical properties of the biofilm, NO-releasing chitosan oligosaccharides exhibited dose-dependent behavior with biofilm degradation. Control chitosan oligosaccharides increased biofilm elasticity, indicating that the scaffold may mitigate the biofilm disrupting power of nitric oxide somewhat. The results from this study indicate that nitric oxide-releasing chitosan oligosaccharides act as dual-action therapeutics capable of eradicating and physically disrupting P. aeruginosa biofilms.