Polaribacter reichenbachii sp. nov.: A New Marine Bacterium Associated with the Green Alga Ulva fenestrata
Polaribacter reichenbachii sp. nov.: A New Marine Bacterium Associated with the Green Alga Ulva fenestrata
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DOI：
10.1007/s00284-012-0200-x
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发表时间：
2012-09
影响因子：
2.6
通讯作者：
O. Nedashkovskaya;A. D. Kukhlevskiy;N. Zhukova
中科院分区：
文献类型：
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作者：
O. Nedashkovskaya;A. D. Kukhlevskiy;N. Zhukova
从常见的太平洋绿色海藻孔石莼（Ulva fenestrata）中分离到一株革兰氏阴性、需氧、杆状、游动、黄色的细菌，命名为6Alg 8 T。基于16 S rRNA基因序列的系统发育分析表明，该菌株属于拟杆菌门（Bacteroidetes）黄杆菌科（Flavobacteriaceae）的Polaribacter属，与Polaribacter dokdonensis DSW-5 T的同源性为97.6%，与其它已知的Polaribacter属的同源性为92.8- 96.1%。菌株6Alg 8 T的主要脂肪酸为iso-C15：0、iso-C15：1、iso-C15：02-OH、C15：0和C15：1ω6。极性脂质谱由主要脂质磷脂酰乙醇胺、两种未知氨基脂质和一种未知脂质组成。模式菌株的DNA G+C含量为31.6mol%。新的分离物和模式菌株的确认种的属Polariacidae很容易区分的基础上的一些表型特征。基因型和表型数据的结合表明，藻类分离物代表了一种新的Polaribacter属物种，其名称为Polaribacter reichenbachiisp。十一是建议。模式菌株为6Alg 8 T（= KCTC 23969 T = KMM 6386 T = LMG 26443 T）。
A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 6Alg 8T, was isolated from the common Pacific green algaUlva fenestrata. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genusPolaribacter, a member of the familyFlavobacteriaceae, the phylumBacteroidetes, with sequence similarities of 97.6 % toPolaribacter dokdonensisDSW-5Tand 92.8–96.1 % to other recognizedPolaribacterspecies. The prevalent fatty acids of strain 6Alg 8Twere iso-C15:0, iso-C15:1, iso-C15:02-OH, C15:0and C15:1ω6. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The DNA G+C content of the type strain is 31.6 mol%. The new isolate and the type strains of recognized species of the genusPolaribacterwere readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genusPolaribacter, for which the namePolaribacter reichenbachiisp. nov. is proposed. The type strain is 6Alg 8T(= KCTC 23969T= KMM 6386T= LMG 26443T).