Antibacterial Copper-Hydroxyapatite Composite Coatings via Electrochemical Synthesis
Antibacterial Copper-Hydroxyapatite Composite Coatings via Electrochemical Synthesis
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DOI：
10.1021/acs.langmuir.9b00919
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发表时间：
2019-04-30
期刊：
影响因子：
3.9
通讯作者：
Yates, Matthew Z.
中科院分区：
文献类型：
--
作者：
Ghosh, Rashmi;Swart, Oliver;Yates, Matthew Z.
采用连续两个电化学反应合成了钛表面的抗菌铜-羟基磷灰石（Cu-HA）复合涂层。第一阶段，采用阴极电解法制备羟基磷灰石纳米晶。然后在第二反应阶段以ha包覆的钛作为阴极，电化学还原溶液中的Cu2+离子为金属Cu纳米粒子。结果表明，反应条件可以在透明质酸晶体表面生成纳米级的Cu颗粒。两阶段合成可以很容易地控制HA涂层中的铜含量。抑菌活性是通过培养大肠杆菌（革兰氏阴性）和金黄色葡萄球菌（革兰氏阳性）在具有不同铜含量的涂层存在。该涂层对两种细菌的抑菌活性与铜浓度有关，这可能是由于铜离子从涂层中缓慢释放所致。观察到生物活性HA支架上相对低负荷的铜的抗菌活性表明，多功能种植体涂层可以补充或替代种植手术中使用的预防性抗生素，这些抗生素负责产生耐药细菌菌株。
Antibacterial copper-hydroxyapatite (Cu-HA) composite coatings on titanium were synthesized using a novel process consisting of two consecutive electrochemical reactions. In the first stage, HA nanocrystals were grown on titanium using the cathodic electrolytic synthesis. The HA-coated titanium was then used as the cathode in a second reaction stage to electrochemically reduce Cu2+ ions in solution to metallic Cu nanoparticles. Reaction conditions were found that result in nanoscale Cu particles growing on the surface of the HA crystals. The two-stage synthesis allows facile control of copper content in the HA coatings. Antibacterial activity was measured by culturing Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) in the presence of coatings having varying copper contents. The coatings displayed copper concentration-dependent antibacterial activity against both types of bacteria, likely due to the slow release of copper ions from the coatings. The observation of antibacterial activity from a relatively low loading of copper on the bioactive HA support suggests that multifunctional implant coatings can be developed to supplement or supplant prophylactic antibiotics used in implant surgery that are responsible for creating resistant bacteria strains.