Ciliogenesis in the mouse oviduct. A scanning electron microscope study.
Ciliogenesis in the mouse oviduct. A scanning electron microscope study.
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DOI：
10.1083/jcb.62.3.899
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发表时间：
1974-09
期刊：
影响因子：
--
通讯作者：
Dirksen ER
中科院分区：
文献类型：
--
作者：
Dirksen ER
小鼠输卵管是一个复杂的盘绕管，可以方便地分为三个不同的区域：伞部、壶腹和峡部。漏斗状的流苏尖端是输卵管纤毛最丰富的部分，排卵后卵子通过它运输。在成体中，纤毛的功能是将卵子运输到伞状尖端的孔中，并从那里运输到壶腹区域，在那里发生受精 (4)。纤毛细胞中散布着一些非纤毛细胞，其功能似乎是分泌性的 (15)。在输卵管发育过程中，纤毛细胞分化表现为每个细胞表面产生大量纤毛。尽管小鼠输卵管上纤毛的广泛形成仅在出生后开始，并持续约 12 天，或直到大多数上皮细胞完全纤毛；一些纤毛细胞在出生时就已经存在。输卵管表面纤毛细胞的形成在空间和时间上都是随机的。然而，纤毛以某种有序的模式出现在每个单独细胞的表面上。这些通过扫描电子显微镜 (SEM) 获得的结果为纤毛细胞分化和纤毛形态发生过程中发生的复杂事件序列的描述添加了更多细节，而这些事件是通过透射电子显微镜无法从之前的研究中预测到的 (5,6,7,13,16,21,23)。扫描电子显微镜可以避免薄切片技术带来的限制，可以同时观察大的表面区域。
The mouse oviduct is a complex, coiled tube which can be conveniently divided into three distinct regions: fimbria, ampulla, and isthmus. The funnel-like fimbriated tip, through which the ovum is transported after ovulation, is the most heavily ciliated portion of the oviduct. In the adult the cilia function to transport ova into the ostium of the fimbriated tip, and from there into the ampullary region, where fertilization occurs (4). Interspersed among the ciliated ceils are a few nonciliated cells whose function appears to be secretory (15). During the development of the oviduct, ciliated cell differentiation is manifested by the production of large numbers of cilia on the surface of each cell. Although extensive formation of cilia on the mouse oviduct begins only after birth and continues for approximately 12 days, or until most of the cells of the epithelium are fully ciliated; a few ciliated cells are already present at birth. The formation of ciliated cells is random over the surface of the oviduct both in space and in time. However, cilia emerge in a somewhat ordered pattern on the surface of each individual cell. These results, obtained by scanning electron microscopy (SEM), add additional details to the description of the complex sequence of events which occur during ciliated cell differentiation and ciliary morphogenesis which could not have been predicted from previous studies by transmission electron microscopy (5, 6, 7, 13, 16, 21, 23). The limitations imposed by thin-sectioning techniques can be avoided in scanning electron microscopy, where large surface areas can be viewed simultaneously.