Tomato SD1, encoding a kinase-interacting protein, is a major locus controlling stem development.
Tomato SD1, encoding a kinase-interacting protein, is a major locus controlling stem development.
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番茄 SD1 编码激酶相互作用蛋白，是控制茎发育的主要基因座
DOI：
10.1093/jxb/eraa144
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发表时间：
2020
影响因子：
6.9
通讯作者：
Zhang Yuyang
中科院分区：
文献类型：
--
作者：
Ye Jie;Tian Ranwen;Meng Xiangfei;Tao Peiwen;Li Changxing;Liu Genzhong;Chen Weifang;Wang Ying;Li Hanxia;Ye Zhibiao;Zhang Yuyang
茎是植物发育的关键决定因素，它连接和支撑植物体的各个部分，运输对影响作物产量的长距离通讯至关重要的营养物质，并产生新的器官。然而，关于作物茎发育调控的研究相当有限。在这里，我们发现番茄（Solanum lycopersicum）茎直径（SD）和果实大小之间存在显著相关性（P<0.001）。我们进行了全基因组关联研究，并确定了一个新的数量性状位点（QTL），SDR 9（9号染色体上的茎直径调节器），共定位与激酶相互作用家族蛋白（KIP）编码的基因，这是最有可能的候选基因SD（以下简称为SD 1）。在细茎材料中过量表达SD 1导致SD增加。与此相反，抑制表达的SD 1在粗茎加入RNA干扰表现出相反的效果。进一步的显微分析表明，SD 1通过控制次生韧皮部细胞的大小和数量来影响茎的直径。在SD 1的启动子区有一个11-bp的插入缺失，它破坏了一个赤霉素反应元件。表达分析表明，SD 1主要在茎的形成层表达，对茎的发育起正调控作用。进化分析表明，SD 1的粗茎等位基因是在番茄的近期改良过程中被选择的。我们的研究结果提供了新的遗传和分子洞察自然变异的SD在番茄和可能加速育种高产番茄。
Stems serve as key determinants of plant development by connecting and supporting parts of the plant body, transporting nutrients important for long-distance communication that affect crop yield, and producing new organs. Nonetheless, studies on the regulation of stem development in crops are rather limited. Here, we found a significant correlation (P<0.001) between stem diameter (SD) and fruit size in tomato (Solanum lycopersicum). We performed a genome-wide association study and identified a novel quantitative trait locus (QTL),SDR9(stem diameter regulator on CHROMOSOME 9), that co-localized with a gene encoding a kinase-interacting family protein (KIP), which is the most likely candidate gene related to SD (hereafter referred to asSD1). Overexpression ofSD1in thin-stem accessions resulted in increased SD. In contrast, suppressed expression ofSD1in thick-stem accessions using RNA interference exhibited the opposite effect. Further microscopic analyses showed thatSD1affected the stem diameter by controlling the size and number of secondary phloem cells. An 11-bp indel in the promoter region ofSD1that disrupts a gibberellin-responsivecis-element was linked to SD. Expression analysis revealed thatSD1was mainly expressed at the cambium of the stem and positively regulates stem development. Evolutionary analysis revealed that the thick-stem allele ofSD1was selected during the recent process of tomato improvement. Our results provide novel genetic and molecular insight into natural variation of SD in tomato and may accelerate the breeding of high yield tomato.