SOME SOURCES OF ERROR IN MEASURING REGIONAL BLOOD FLOW WITH RADIOACTIVE MICROSPHERES
SOME SOURCES OF ERROR IN MEASURING REGIONAL BLOOD FLOW WITH RADIOACTIVE MICROSPHERES
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DOI：
10.1152/jappl.1971.31.4.598
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发表时间：
1971-01-01
影响因子：
3.3
通讯作者：
FIXLER, DE
中科院分区：
文献类型：
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作者：
BUCKBERG, GD;LUCK, JC;FIXLER, DE
Julien IE霍夫曼，JOSEPH P. ARCHIE，和大卫E.修理工。放射性微球测量局部血流量的误差来源。J.Appl.Physiol.31（4）：538-604. 1971.放射性微球（直径8-50 μ m）与局部血流成比例分布，因此它们在所有动脉中的浓度应该相似，但随机变化。将不同数量的微球注射到羔羊、绵羊和犬的左心房和心室中，并在同时从两个或多个动脉（股动脉、颈动脉或主动脉）、左心室和右心室的游离壁以及两个肾脏中抽取的样品中测量微球浓度。冠状动脉和肾血流量测量微球（使用外周参考样本）和通过独立的方法。尽管有一些nonrandom（混合不良）的错误通常在20%以下的所有注射和采样地点，在这两个物种，只要每个样品有超过400个微球;较少的数字造成的错误高达113 y 0。犬的变异性小于绵羊，左心房注射的变异性小于绵羊，但所有动脉的变异性相同。冠状动脉和肾脏流量的微球通常分别在20 y 0和6%，测量流量。最大精密度发生在注射最大数量的微球时，与无生理变化一致。二项分布泊松变量;冠状动脉血流量;肾血流量;左心房注射;左心室注射最近Rudolph和Heymann（8）介绍了一种用直径为50 μ m的放射性微球测量局部血流量的方法。这些被注射到循环中，并行进到小的外周血管，在那里它们被捕获;然后取出器官并测量它们的放射性。重复测量可以通过使用不同的核素并通过伽马能谱法分离它们来进行。在几项研究中，他们和其他工作人员（2，3，5，8）表明，在各个器官中的总放射性分数与球体通过期间到达这些器官的心输出量分数相似，但方法的可变性和造成这种可变性的因素尚未完全确定。
JULIEN IE HOFFMAN, JOSEPH P. ARCHIE, AND DAVID E. FIXLER. Some sources of error in measuring regional blood flow with radioactive microspheres. J. Appl. Physiol. 3 l (4): 538-604. 197 1.-Radioactive microspheres(8-50 p diameter) distribute in proportion to regional blood flow so that their concentrations in all arteries should be similar but for random variation. Varying numbers of microspheres were injected into the left atrium and ventricles of lambs, sheep, and dogs, and microsphere concentrations were measured in samples drawn simultaneously from two or more arteries (femoral, carotid, or aorta), in the free walls of the left and right ventricles and both kidneys. Coronary and renal blood flows were measured with microspheres(using peripheral reference samples) and by independent methods. Despite some nonrandomness (poor mixing) errors were usually under 20% for all injection and sampling sites in both species as long as each sample had over 400 microspheres; lesser numbers caused errors up to 1 13y0. Variability was less in dogs than sheep, less for left atria1 injections, but equivalent in all arteries. Coronary and renal flows by microspheres were usually within 20y0 and 6%, respectively, of measured flow. The greatest precision occurs on injection of the largest number of microspheres consistent with no physiological changes. binomial distribution; Poisson variate; coronary blood flow; renal blood flow; left atria1 injection; left ventricular injectionRECENTLY RUDOLPH AND HEYMANN(8) introduced a method of measuring regional blood flow with radioactive micro-spheres 50 p in diameter. These were injected into the cir-culation and traveled to the small peripheral vessels where they were trapped; the organs were then removed and their radioactivity measured. Repeated measurements could be made by using different nuclides and separating them by gamma spectrometry. In several studies they and other workers (2, 3, 5, 8) showed that the fraction of total radioactiyity in various organs was similar to the fraction of cardiac output reaching those organs during the transit of the spheres, but the variability of the method and the factors responsible for that variability have not yet been fully defined.