A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening
A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening
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DOI：
10.1073/pnas.94.5.1914
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发表时间：
1997-03-04
影响因子：
11.1
通讯作者：
Old, LJ
中科院分区：
文献类型：
--
作者：
Chen, YT;Scanlan, MJ;Old, LJ
利用肿瘤mRNA和自体患者血清对重组cDNA表达文库（SEREX）进行血清学分析，为鉴定免疫原性肿瘤抗原提供了强有力的方法。我们将该方法应用于一例食管鳞状细胞癌，并鉴定了几个候选肿瘤靶点，其中NY-ESO-1在正常组织中表达有限，仅在睾丸和卵巢组织中表达高水平。逆转录- pcr分析显示NY- ESO-1 mRNA在多种人类癌症中表达，包括黑色素瘤、乳腺癌、膀胱癌、前列腺癌和肝细胞癌，NY ESO-1编码Mr 17,995的推定蛋白，与任何已知蛋白无同源性。NY-ESO-1的表达模式表明，它属于一个不断扩大的免疫原性睾丸抗原家族，这些抗原在人类癌症中以谱系非特异性方式异常表达，这些抗原最初由细胞毒性T细胞（MAGE， BAGE, GAGE-1）或抗体[homm - mel -40(SSX2)， NY-ESO-1]检测，代表了癌症疫苗接种的抗原靶点库。
Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens, We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets, One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues, Reverse transcription-PCR analysis showed NY-ESO-I mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma, NY ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein, The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage-nonspecific fashion, These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination.