Assessment of Vascular Patterning in the Zebrafish.
Assessment of Vascular Patterning in the Zebrafish.
复制标题
斑马鱼血管模式的评估。
DOI：
10.1007/978-1-0716-0916-3_15
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Weinstein,BrantM
中科院分区：
文献类型：
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作者：
Stratman,AmberN;Weinstein,BrantM
斑马鱼已成为研究血管发育和血管生物学的重要模式生物。在这里，我们讨论了一些用于研究鱼类血管的方法，包括功能丧失的工具，如morpholinos和遗传突变体，沿着评估血管表型的方法和注意事项。我们还提供了详细的协议，用于斑马鱼血管系统的重要成像方法，包括微血管造影和长期延时成像。我们描述的方法，我们建议使用这些方法观察到的表型评估的考虑因素，将有助于确保可靠的，有效的结论时，评估血管表型遗传或实验操作的斑马鱼。
The zebrafish has emerged as a valuable and important model organism for studying vascular development and vascular biology. Here, we discuss some of the approaches used to study vessels in fish, including loss-of-function tools such as morpholinos and genetic mutants, along with methods and considerations for assessing vascular phenotypes. We also provide detailed protocols for methods used for vital imaging of the zebrafish vasculature, including microangiography and long-term time-lapse imaging. The methods we describe, and the considerations we suggest using for assessing phenotypes observed using these methods, will help ensure reliable, valid conclusions when assessing vascular phenotypes following genetic or experimental manipulation of zebrafish.