Expression of ST2, an interleukin-1 receptor homologue, is induced by proinflammatory stimuli
Expression of ST2, an interleukin-1 receptor homologue, is induced by proinflammatory stimuli
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DOI：
10.1006/bbrc.1997.6810
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发表时间：
1997-06-27
影响因子：
3.1
通讯作者：
Young, PR
中科院分区：
文献类型：
--
作者：
Kumar, S;Tzimas, MN;Young, PR
ST 2/T1是与IL-1受体高度同源的孤儿受体。使用ST 2 cDNA，ST 2特异性引物，和针对ST 2产生的多克隆抗体，mRNA和蛋白质的表达相应的可溶性和膜锚定形式的ST 2进行了研究。ST 2 mRNA在所有检查的人体组织中普遍表达，并由细胞因子和佛波醇酯诱导。在不同的人类细胞和组织中观察到三种不同种类的mRNA。而在Balb/c-3 T3细胞中仅检测到两种ST 2 mRNA，而在正常小鼠的大部分组织中均未检测到ST 2 mRNA。然而，在小鼠耳暴露于UVB照射导致炎症的小鼠模型中，ST 2 mRNA在UV暴露后48小时表达。类似地，在Balb/c-3 T3细胞中，可溶性ST 2 mRNA和蛋白质的表达在指数生长和静止细胞中均由促炎刺激物如TNF、IL-1 α、IL-1 β和PMA诱导。然而，膜ST 2的表达保持恒定。这些数据表明ST 2在炎症中的作用。(C)北京：科学出版社.
ST2/T1 is an orphan receptor highly homologous to the IL-1 receptor. Using ST2 cDNA, ST2 specific primers, and a polyclonal antibody generated against ST2, the expression of mRNA and protein corresponding to both the soluble and membrane anchored forms of ST2 was studied. ST2 mRNAs were ubiquitously expressed in all the human tissues examined and were induced by cytokines and phorbol esters. Three different species of mRNAs were observed in different human cells and tissues. In contrast, only two species of ST2 mRNAs were observed in murine Balb/c-3T3 cells and no ST2 mRNA was seen in most tissues of normal mice. However, in a murine model where mouse ears are exposed to UVB irradiation leading to inflammation, ST2 mRNA was expressed 48 h post UV exposure. Similarly, in Balb/c-3T3 cells, the expression of soluble ST2 mRNA and protein was induced by pro-inflammatory stimuli such as TNF, IL-1 alpha, IL-1 beta and PMA in both exponentially growing and quiescent cells. The expression of the membrane ST2, however, remained constant. These data suggest a role for ST2 in inflammation. (C) 1997 Academic Press.