Essential role of the small GTPase Rac in disease resistance of rice
Essential role of the small GTPase Rac in disease resistance of rice
复制标题
DOI：
10.1073/pnas.021273498
复制
发表时间：
2001-01-16
影响因子：
11.1
通讯作者：
Shimamoto, K
中科院分区：
文献类型：
--
作者：
Ono, E;Wong, HL;Shimamoto, K
活性氧中间体（ROI）的产生和一种称为超敏反应（HR）的程序性细胞死亡形式通常与植物的抗病性有关。我们之前已经证明，水稻的Pac同源物OsRac1是水稻ROI产生和细胞死亡的调节因子。在这里，我们表明，组成活性OsRac1 (i)引起hr样反应，并大大减少了对稻瘟病菌剧毒种的疾病损害；（ii）引起对细菌性枯萎病毒种的抗性；（iii）导致植物抗毒素的产生增强，并改变防御相关基因的表达。在转基因细胞培养中，显性阴性的OsRac1抑制激发子诱导的ROI产生，在植物中抑制真菌无毒种诱导的HR。综上所述，我们的研究结果强烈表明OsRac1在水稻抗病中具有普遍作用。
Production of reactive oxygen intermediates (ROI) and a form of programmed cell death called hypersensitive response (HR) are often associated with disease resistance of plants. We have previously shown that the Pac homolog of rice, OsRac1, is a regulator of ROI production and cell death in rice. Here we show that the constitutively active OsRac1 (i) causes HR-like responses and greatly reduces disease lesions against a virulent race of the rice blast fungus; (ii) causes resistance against a Virulent race of bacterial blight; and (iii) causes enhanced production of a phytoalexin and alters expression of defense-related genes. The dominant-negative OsRac1 suppresses elicitor-induced ROI production in transgenic cell cultures, and in plants suppresses the HR induced by the avirulent race of the fungus. Taken together, our findings strongly suggest that OsRac1 has a general role in disease resistance of rice.