Mate choice and gene expression signatures associated with nutritional adaptation in the medfly ( Ceratitis capitata )
Mate choice and gene expression signatures associated with nutritional adaptation in the medfly ( Ceratitis capitata )
复制标题
与地中海果蝇（Ceratitis headata）营养适应相关的配偶选择和基因表达特征
DOI：
10.1101/362210
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nash W
中科院分区：
文献类型：
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作者：
Nash W
对营养的进化反应是理解宿主转移和由此产生的生殖隔离潜力的关键。实验进化以前已被用来描述地中海实蝇（Ceratitis capitata）幼虫的饮食与不同的营养特性的反应。在30代内，这导致了幼虫发育时间、卵至成虫存活和成虫体型适应的差异。在这里，我们使用mRNA-seq来确定这些相同人群中基因表达模式的差异，使用来自第60代营养选择的男性。我们通过qRT-PCR验证了差异表达，发现与代谢、氧化磷酸化和蛋白水解相关的基因在差异表达基因中显着过多。这些结果提供了第一个全基因组调查的假定机制的基础上进化的反应，营养适应。此外，我们测试了营养适应可以改变交配模式的假设。我们发现证据表明，通过饮食在第60代，但不是90。因此，该模式在各代之间是可变的，并且总体上没有证据表明品系之间存在任何孤立的交配分歧。总的来说，这些结果提供了对饮食适应机制的深入了解，并扩展了我们对哪些性状代表营养选择的核心反应的认识。
Evolutionary responses to nutrition are key to understanding host shifts and the resulting potential for reproductive isolation. Experimental evolution has previously been used to describe the responses of the medfly (Ceratitis capitata) to larval diets with different nutritional properties. Within 30 generations this led to divergence in larval development time, egg to adult survival and adaptation in adult body size. Here we used mRNA-seq to identify differences in gene expression patterns in these same populations, using males from the 60thgeneration of nutritional selection. We validated differential expression by using qRT-PCR and found that genes linked to metabolism, oxidative phosphorylation and proteolysis were significantly over-represented among the differentially expressed genes. The results provide the first genome-wide survey of the putative mechanisms underpinning evolved responses to nutritional adaptation. In addition, we tested the hypothesis that nutritional adaptation can alter mating patterns. We found evidence for assortative mating by diet at generation 60, but not 90. Hence, the pattern was variable across generations and there was no evidence overall for any isolating mating divergence between the lines. Overall, the results provide insight into the mechanisms underpinning dietary adaptation and extend our knowledge of which traits represent core responses to nutritional selection.