Anthocyanin Accumulation and Molecular Analysis of Anthocyanin Biosynthesis-Associated Genes in Eggplant (Solanum melongena L.)
Anthocyanin Accumulation and Molecular Analysis of Anthocyanin Biosynthesis-Associated Genes in Eggplant (Solanum melongena L.)
复制标题
茄子花青素积累及花青素生物合成相关基因的分子分析（Solanum melongena L.）
DOI：
10.1021/jf404574c
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发表时间：
2014-04-02
影响因子：
6.1
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
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作者：
Zhang, Yanjie;Hu, Zongli;Chen, Guoping
茄子（Solanum melongena L.）是一种在世界范围内种植和消费的食用水果蔬菜。紫色茄子因其果皮中含有的有益健康的花青素而更加引人注目，备受欢迎。采用高效液相色谱-电喷雾串联质谱法分离鉴定了紫昌中的两种花色苷。为探讨茄子花青苷积累的分子机制，对紫色茄子和白色茄子（白雪）果皮和果肉中花青苷生物合成和调控基因的转录本进行了分析。与其他组织相比，SmMYB 1和除PAL外的所有花青素生物合成基因在紫色品种的果皮中显著上调。SmMYB 1的过表达激活了茄子再生芽中大量花青素的积累。这些结果证明，SmMYB 1的转录激活占大多数花色苷生物合成基因的组成性上调和紫色品种花色苷生物合成的开始。
Eggplant (Solanum melongena L.) is an edible fruit vegetable cultivated and consumed worldwide. The purple eggplant is more eye-catching and popular for the health-promoting anthocyanins contained in the fruit skin. Two kinds of anthocyanin were separated and identified from purple cultivar (Zi Chang) by high-performance liquid chromatography electrospray ionization tandem mass spectrometry. To investigate the molecular mechanisms of anthocyanin accumulation in eggplant, the transcripts of anthocyanin biosynthetic and regulatory genes were analyzed in the fruit skin and the flesh of the purple cultivar and the white cultivar (Bai Xue). Compared with the other tissues, SmMYB1 and all anthocyanin biosynthetic genes except PAL were dramatically upregulated in the fruit skin of the purple cultivar. Overexpression of SmMYB1 activated abundant anthocyanin accumulation in the regenerating shoots of eggplant. These results prove that transcriptional activation of SmMYB1 accounts for constitutive upregulation of most anthocyanin biosynthetic genes and the onset of anthocyanin biosynthesis in the purple cultivar.