Role of endothelium and hypercholesterolemia in intimal thickening and lipid accumulation.
Role of endothelium and hypercholesterolemia in intimal thickening and lipid accumulation.
复制标题
内皮和高胆固醇血症在内膜增厚和脂质积累中的作用。
DOI：
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发表时间：
1979
影响因子：
6
通讯作者：
William Insull
中科院分区：
文献类型：
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作者：
C. Minick;M. B. Stemerman;William Insull
在本文报道的实验中，我们验证了内皮持续缺失有利于内膜增厚、脂质堆积和动脉粥样硬化的假说。第0天用球囊导管去除兔主动脉内皮细胞。最初，所有的兔子都被喂以低脂饮食。部分兔(I组)在去内皮化后继续低脂饮食8~20周。从去内皮化后4~9周开始，分别饲喂半合成高脂饲料(II组)或高胆固醇饲料(III组)4~20周。在所有组中，再内皮化的血管内膜中的脂质堆积显著大于邻近无内皮层的内膜。在I组、II组和III组的主动脉中，再内皮化区的内膜增厚程度明显大于邻近无内皮区。与正常胆固醇血症组相比，高胆固醇血症组兔再内皮化区内膜厚度明显增加，而内皮缺损区内膜厚度无明显变化。因此，这些实验的结果并不支持这样的假设，即内皮缺失特别有利于内膜增厚和内膜脂质堆积。结果表明，新生内皮复盖的血管内膜明显增厚，更容易积聚脂质。
In experiments reported here we tested the hypothesis that persistent absence of endothelium favors intimal thickening, lipid accumulation, and atherosclerosis. Rabbit aortas were de-endothelialized with a balloon catheter at Day 0. Initially, all rabbits were fed a diet low in lipid. Some rabbits (Group I) were continued on a diet low in lipid for 8 to 20 weeks after de-endothelialization. Beginning 4 to 9 weeks after de-endothelialization, other rabbits were fed semisynthetic lipid-rich diets (Group II) or cholesterol-supplemented diets (Groups III) for 4 to 20 weeks. Lipid accumulation in all groups was significantly greater in the re-endothelialized intima than in adjacent intima lacking an endothelial lining. In aortas of Groups I, II, and III the degree of intimal thickening was significantly greater in re-endothelialized areas than in adjacent areas lacking endothelium. Intimal thickness was enhanced in re-endothelialized areas of hypercholesterolemic rabbits of Group III compared with normocholesterolemic rabbits of Group I but not in areas lacking endothelium. Thus, results of these experiments do not support the hypothesis that the absence of endothelium particularly favors intimal thickening and intimal lipid accumulation. Results indicate that intima covered by regenerated endothelium is significantly thicker and more likely to accumulate lipid.