CHARACTERIZATION OF PHOTOSYSTEM-II MUTANTS OF CHLAMYDOMONAS-REINHARDII LACKING THE PSBA GENE
CHARACTERIZATION OF PHOTOSYSTEM-II MUTANTS OF CHLAMYDOMONAS-REINHARDII LACKING THE PSBA GENE
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DOI：
10.1007/bf00021484
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发表时间：
1986-01-01
影响因子：
5.1
通讯作者：
ROCHAIX, JD
中科院分区：
文献类型：
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作者：
BENNOUN, P;SPIERERHERZ, M;ROCHAIX, JD
我们检查了 78 个缺乏光系统 II 活性的莱茵衣藻叶绿体突变体。它们中的大多数无法合成 32 Kdalton 蛋白质。对其中 22 个突变体的分析表明，它们的叶绿体基因组中删除了 psbA 基因（编码 32 Kdalton 蛋白）的两个拷贝。尽管这些突变体能够合成并整合其他光系统II多肽到类囊体膜中，但它们无法组装稳定的功能性光系统II复合物。因此，32 K 蛋白似乎不仅在光系统 II 功能中发挥着重要作用，而且在稳定该复合物方面也发挥着重要作用。
We have examined 78 chloroplast mutants of Chlamydomonas reinhardii lacking photosystem II activity. Most of them are unable to synthesize the 32 Kdalton protein. Analysis of 22 of these mutants reveals that they have delected both copies of the psbA gene (which codes for the 32 Kdalton protein) in their chloroplast genome. Although these mutants are able to synthesize and to integrate the other photosystem II polypeptides in the thylakoid membranes, they are unable to assemble a stable functional photosystem II complex. The 32 Kprotein appears therefore to play an important role not only in photosystem II function, but also in stabilizing this complex.