Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots
Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots
复制标题
DOI：
10.1016/j.jcv.2010.02.006
复制
发表时间：
2010-05-01
影响因子：
8.8
通讯作者：
Sherman, G. G.
中科院分区：
文献类型：
--
作者：
Lilian, R. R.;Bhowan, K.;Sherman, G. G.
背景：南非更多的常规实验室具备执行定量而非定性 HIV 病毒检测分析的能力。如果对干血斑 (DBS) 进行定量病毒载量 (VL) 检测能够准确诊断 HIV 感染，那么早期婴儿诊断的可及性将会得到提高。该国常规使用的 VL 检测此前尚未在 DBS 上进行过婴儿早期诊断评估。 目的： 确定 DBS 上的 NucliSens EasyQ 检测（法国里昂生物梅里埃）对 C 亚型感染人群中 HIV 早期婴儿诊断的准确性。 研究设计： 对从进行 HIV 检测的儿童收集的存储 DBS 样本进行了分析。将 DBS EasyQ VL 结果与通过全血 HIV DNA PCR 结果确定的儿童 HIV 状态进行比较。结果：EasyQ 检测对来自 71 名 HIV 感染者和 164 名 HIV 未感染儿童的 235 份 DBS 储存样本进行了分析。在接受检测的 216 名婴儿（12 个月或以下儿童）中，全部 52 名 HIV 感染婴儿均被检出（灵敏度为 100%）。 164 名未感染 HIV 的婴儿中有 6 名得出假阳性结果（特异性 96.3%）。所有假阳性婴儿和六名真阳性婴儿均患有 VL
Background: More routine laboratories in South Africa are equipped to perform quantitative than qualitative HIV viral detection assays. The accessibility of early infant diagnosis would be improved if a quantitative viral load (VL) assay performed on dried blood spots (DBS) could accurately diagnose HIV-infection. The VL assay routinely used in the country has not previously been assessed on DBS for early infant diagnosis.Objectives: To determine the accuracy of the NucliSens EasyQ assay (bioMerieux, Lyon, France) on DBS for early infant diagnosis of HIV in a subtype C-infected population.Study design: Stored DBS samples collected from children presenting for HIV testing were analyzed. DBS EasyQ VL results were compared to the child's HIV status as determined by a whole blood HIV DNA PCR result.Results: The EasyQ assay was performed on 235 stored DBS samples from 71 HIV-infected and 164 HIV-uninfected children. Of the 216 infants (children aged 12 months or less) tested, all 52 HIV-infected infants were detected (sensitivity of 100%). Six of 164 HIV-uninfected infants yielded false positive results (specificity 96.3%). All false positive and six of the true positive infants had VL