The bacterial replisome: back on track?
The bacterial replisome: back on track?
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DOI：
10.1111/j.1365-2958.2008.06378.x
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发表时间：
2008-09-01
影响因子：
3.6
通讯作者：
Bates, David
中科院分区：
文献类型：
--
作者：
Bates, David
据推测，细菌DNA复制通过工厂机制发生，其中未复制的DNA被缠绕到位于中心的复制体中，而新合成的DNA被排放到相反的细胞两极。虽然这一观点得到了相当多的支持，但它与许多关键意见不符。我回顾了新的发现，并为旧的发现提供了不同的解释，这对这个模型提出了挑战。总的来说，目前的数据表明，复制体，至少在缓慢生长的大肠杆菌细胞中，沿着沿着一条静止的染色体。这些复制体在运动中不是静止的、被束缚的或受限制的，而是在整个类核中旅行。一种可能性是复制体沿着由环状结构域组成的染色体导航，正如之前所设想的那样。
It has been postulated that bacterial DNA replication occurs via a factory mechanism in which unreplicated DNA is spooled into a centrally located replisome and newly synthesized DNA is discharged towards opposite cell poles. Although there is considerable support for this view, it does not fit with many key observations. I review new findings, and provide alternative interpretations for old findings, which challenge this model. As a whole, current data suggest that the replisome, at least in slowly growing Escherichia coli cells, tracks along a stationary chromosome. These replisomes are not stationary, tethered or restricted in their movement, but rather travel throughout the nucleoid. One possibility is that the replisome navigates along a chromosome made up of looped domains as has been previously envisioned.