The Characterization of chIFITMs in Avian Coronavirus Infection In Vivo, Ex Vivo and In Vitro
The Characterization of chIFITMs in Avian Coronavirus Infection In Vivo, Ex Vivo and In Vitro
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DOI：
10.3390/genes11080918
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发表时间：
2020-08
期刊：
影响因子：
3.5
通讯作者：
Angela Steyn;Sarah Keep;Erica Bickerton;M. Fife
中科院分区：
文献类型：
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作者：
Angela Steyn;Sarah Keep;Erica Bickerton;M. Fife
冠状病毒是一大类包膜RNA病毒，通常会在被感染的宿主中引起胃肠道或呼吸道疾病。禽冠状病毒传染性支气管炎病毒（IBV）是鸡的一种高度传染性呼吸道病原体，可影响肾脏和生殖系统，导致禽类死亡和繁殖能力下降。干扰素诱导的跨膜（IFITM）蛋白在病毒感染时被激活，代表了一类限制许多病毒病原体复制的细胞限制因子。本研究采用致病性M41-CK菌株、肾致病性QX菌株和非致病性Beaudette菌株，在体内、离体和体外对IBV感染时鸡IFITM基因的相对mRNA表达进行了表征。在体内，我们证明了M41-CK-和qx感染的气管中chIFITM1、2、3和5在感染两天后显著上调。Beaudette、M41-CK和QX体外感染可导致chIFITM1、2和3在感染后24小时显著上调。我们证实了不同IBV菌株感染后的不同先天反应，并相信我们的数据为chIFITMs在早期IBV感染中的可能作用提供了新的见解。
The coronaviruses are a large family of enveloped RNA viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. Avian coronavirus infectious bronchitis virus (IBV) is a highly contagious respiratory pathogen of chickens that can affect the kidneys and reproductive systems resulting in bird mortality and decreased reproductivity. The interferon-inducible transmembrane (IFITM) proteins are activated in response to viral infections and represent a class of cellular restriction factors that restrict the replication of many viral pathogens. Here, we characterize the relative mRNA expression of the chicken IFITM genes in response to IBV infection, in vivo, ex vivo and in vitro using the pathogenic M41-CK strain, the nephropathogenic QX strain and the nonpathogenic Beaudette strain. In vivo we demonstrate a significant upregulation of chIFITM1, 2, 3 and 5 in M41-CK- and QX-infected trachea two days post-infection. In vitro infection with Beaudette, M41-CK and QX results in a significant upregulation of chIFITM1, 2 and 3 at 24 h post-infection. We confirmed a differential innate response following infection with distinct IBV strains and believe that our data provide new insights into the possible role of chIFITMs in early IBV infection.