Eugenol-induced suppression of biofilm-forming genes in Streptococcus mutans: An approach to inhibit biofilms
Eugenol-induced suppression of biofilm-forming genes in Streptococcus mutans: An approach to inhibit biofilms
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DOI：
10.1016/j.jgar.2014.05.006
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发表时间：
2014-12-01
影响因子：
4.6
通讯作者：
Khan, Asad U.
中科院分区：
文献类型：
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作者：
Adil, Mohd;Singh, Kunal;Khan, Asad U.
变形链球菌被充分证明是龋齿的主要病原体。在牙齿表面形成生物膜的能力是这种细菌的主要毒力因素。本研究的目的是评估丁香酚对变形链球菌生物膜和群体感应 (QS) 相关基因的抑制作用，并确定其假定的作用模式。评估了丁子香酚对毒力特性（例如粘附和生物膜形成）的抑制活性。使用共焦激光扫描显微镜和透射电子显微镜分析和观察变形链球菌结构和生物膜的形态变化。通过实时定量 PCR (qRT-PCR) 检查丁子香酚对生物膜和 QS 相关基因（gtfB、gtfC、comDE、smu630、vicR、brpA、ftf、relA、gbpB 和 spaP）表达的影响。目前的数据显示，亚最小抑制浓度（亚MIC）的丁子香酚显着下调了测试基因的表达，但不影响细菌生长。这些结果表明亚 MIC 丁子香酚可以有效抑制毒力基因。因此，结果表明丁子香酚可以抑制龋齿相关的生物膜，并显示出其针对口腔生物膜的治疗潜力。 (C) 2014 年国际感染和癌症化疗协会。由爱思唯尔有限公司出版。保留所有权利。
Streptococcus mutans is well documented as a major aetiological agent of dental caries. The ability to form a biofilm on tooth surfaces is the major virulence factor of this bacterium. The objective of this study was to evaluate the effect of eugenol on suppression of biofilm-and quorum sensing (QS)-related genes of Streptococcus mutans and to determine its putative mode of action. Eugenol was evaluated for its inhibitory activity against virulence properties such as adherence and biofilm formation. Morphological changes in the architecture of S. mutans and in the biofilm were analysed and observed using confocal laser scanning microscopy and transmission electron microscopy. The effects of eugenol on expression of biofilm-and QS-related genes (gtfB, gtfC, comDE, smu630, vicR, brpA, ftf, relA, gbpB and spaP) were checked by quantitative real-time PCR (qRT-PCR). The present data revealed that eugenol at a sub-minimum inhibitory concentration (sub-MIC) significantly downregulated the expression of tested genes but did not affect bacterial growth. These results suggest that a sub-MIC of eugenol can effectively suppress virulence genes. Thus, the results indicated that eugenol can inhibit caries-associated biofilm and showed its therapeutic potential against oral biofilm. (C) 2014 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.