Alteration of redox state of human serum albumin before and after hemodialysis
Alteration of redox state of human serum albumin before and after hemodialysis
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DOI：
10.1159/000082524
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Era, S
中科院分区：
文献类型：
--
作者：
Soejima, A;Matsuzawa, N;Era, S
背景资料：持续性低白蛋白血症是长期维持性血液透析患者死亡的预测因子，尽管心血管疾病仍然是死亡的主要原因。维持性血液透析患者血清抗氧化活性降低可能导致氧化损伤增加，并可能与加速动脉粥样硬化变化有关。研究方法：本研究的目的是通过高效液相色谱法（HPLC）和荧光检测器检测维持性血液透析患者血清白蛋白的氧化还原状态。结果如下：在pH 4.85的Shodex-Asahipak ES-502N柱上，人血清白蛋白的HPLC显示人巯基白蛋白（HMA）和非巯基白蛋白（HNA）的清晰分离，它们分别是人血清白蛋白的还原和氧化形式。维持性血液透析患者的人血清白蛋白HMA组分的平均+/-SD百分比显著低于年龄匹配的正常受试者。血液透析开始后3 - 5 h HMA百分比升高，然后降至低于正常水平。结论：这表明，血清白蛋白可能是维持性血液透析患者的主要细胞外抗氧化剂，血液透析可能通过诱导分子间巯基-二硫键交换反应来挽救血清白蛋白减少。版权所有（C）2004 S. Karger AG，巴塞尔。
Background: Persistent hypoalbuminemia is a predictor of death in long-term maintenance hemodialysis patients, although cardiovascular diseases remain the leading cause of death. A decreased serum antioxidant activity in maintenance hemodialysis patients may contribute to increased oxidative damage, and may be associated with accelerated atherosclerotic changes. Methods: The aim of this study was to examine the redox state of human serum albumin in maintenance hemodialysis patients by high-performance liquid chromatography (HPLC) using a fluorescence detector. Results: HPLC of human serum albumin on a Shodex-Asahipak ES-502N column at pH 4.85 showed a clear resolution of human mercaptalbumin (HMA) and nonmercaptalbumin (HNA), which are the reduced and oxidized forms of human serum albumin, respectively. The mean +/- SD percentage of the HMA fraction of human serum albumin was significantly lower in maintenance hemodialysis patients than in age-matched normal subjects. The percentage of HMA increased 3 - 5 h after starting the hemodialysis and then decreased to subnormal levels. Conclusion: This suggests that serum albumin may be a major extracellular antioxidant in maintenance hemodialysis patients, and that hemodialysis may rescue serum albumin reduction by inducing intermolecular sulfhydryl-disulfide exchange reaction. Copyright (C) 2004 S. Karger AG, Basel.