Dynamics and biological relevance of DNA demethylation in Arabidopsis antibacterial defense
Dynamics and biological relevance of DNA demethylation in Arabidopsis antibacterial defense
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DOI：
10.1073/pnas.1211757110
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发表时间：
2013-02-05
影响因子：
11.1
通讯作者：
Navarro, Lionel
中科院分区：
文献类型：
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作者：
Yu, Agnes;Lepere, Gersende;Navarro, Lionel
DNA甲基化是一种表观遗传标记，它可以沉默转座元件(TES)并重复。虽然DNA甲基化的建立和维持相对较好，但对它们在动植物天然免疫中的动态和生物学相关性却知之甚少。在这里，我们证明了一些TES在拟南芥的抗菌防御过程中被去甲基化并在转录上重新激活。这种作用与关键转录基因沉默因子的下调有关，部分依赖于主动的去甲基化过程。DNA去甲基化限制了细菌病原菌紫丁香假单胞菌在叶片中的繁殖和维管繁殖，相应地，一些免疫反应基因在其启动子区域含有重复序列，受到DNA甲基化的负调控。这项研究提供的证据表明，DNA去甲基化是植物诱导的免疫反应的一部分，潜在地启动了与TES/重复序列相关的一些防御基因的转录激活。
DNA methylation is an epigenetic mark that silences transposable elements (TEs) and repeats. Whereas the establishment and maintenance of DNA methylation are relatively well understood, little is known about their dynamics and biological relevance in plant and animal innate immunity. Here, we show that some TEs are demethylated and transcriptionally reactivated during antibacterial defense in Arabidopsis. This effect is correlated with the down-regulation of key transcriptional gene silencing factors and is partly dependent on an active demethylation process. DNA demethylation restricts multiplication and vascular propagation of the bacterial pathogen Pseudomonas syringae in leaves and, accordingly, some immune-response genes, containing repeats in their promoter regions, are negatively regulated by DNA methylation. This study provides evidence that DNA demethylation is part of a plant-induced immune response, potentially acting to prime transcriptional activation of some defense genes linked to TEs/repeats.