Organ-specific differences in 8-oxoguanosine glycosylase (OGG1) repair following acute treatment with benzo[a]pyrene.
Organ-specific differences in 8-oxoguanosine glycosylase (OGG1) repair following acute treatment with benzo[a]pyrene.
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发表时间：
2001-07
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通讯作者：
T. Stedeford;F. Cardozo‐Pelaez;C. Hover;R. Harbison;J. Sanchez-Ramos
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作者：
T. Stedeford;F. Cardozo‐Pelaez;C. Hover;R. Harbison;J. Sanchez-Ramos
肺已被证明是苯并[a]芘（B[a]P）有害作用的靶器官，无论接触途径如何。8-羟基-2 '-脱氧鸟苷（oxo 8dG）是暴露于B[a] P后在DNA中形成的致突变损伤。用B[a]P处理。将雄性Spraque-Dawley大鼠腹膜内施用20 mg/kg B[a]，2次/天，共5天。在72小时时在肺组织中观察到去除oxo 8dG的能力降低26%，并且在120小时时恢复到高于对照值20%。在分析的所有时间点，肝脏和肾脏的容量均保持在基线水平。在72小时时在肺中观察到oxo 8dG增加7倍。这项研究表明，清除oxo 8dG的能力存在器官特异性差异，并进一步证明了肺组织对B[a] P作用的敏感性。
The lung has been shown to be a target organ for the deleterious effects of Benzo[a]pyrene (B[a]P), regardless of the route of exposure. 8-hydroxy-2'-deoxyguanosine (oxo8dG) is a mutagenic lesion formed in DNA following exposure to B[a]P. The objective of this study was to determine the capacity of different organs to repair oxo8dG following intraperitoneal (i.p.) treatment with B[a]P. Male Spraque-Dawley rats were administered 20 mg/kg B[a]P i.p., 2 times/day for 5 days. A 26% decrease in the capacity to remove oxo8dG was observed in lung tissue at 72 hours and recovered 20% above control values at 120 hours. The capacity of the liver and kidney remained at baseline for all time points analyzed. A 7-fold increase in oxo8dG was observed in the lung at 72 hours. This study demonstrates that organ-specific differences exist in the capacity to remove oxo8dG and further demonstrates the susceptibility of lung tissue to the effects of B[a]P.