MICROINJECTED C-MYC AS A COMPETENCE FACTOR
MICROINJECTED C-MYC AS A COMPETENCE FACTOR
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DOI：
10.1126/science.4001943
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
BASERGA, R
中科院分区：
文献类型：
--
作者：
KACZMAREK, L;HYLAND, JK;BASERGA, R
虽然许多癌基因以细胞周期依赖的方式表达，但它们在控制细胞增殖中的作用只能通过直接功能测定来确定。将c-myc蛋白微量注射到静止瑞士3T3细胞的细胞核中，与血小板缺乏的血浆一起刺激细胞DNA合成。这表明，c-myc蛋白与血小板衍生生长因子（PDGF）一样，可能在细胞周期中作为一种能力因子，促进细胞向S期发展。培养基中存在抗PDGF抗体，可消除微注射PDGF诱导的DNA合成；然而，微注射的c-myc蛋白即使在其自身抗体存在于培养基中时也能刺激DNA合成。c-myc蛋白可能作为细胞内能力因子，而PDGF仅从细胞外表达其生物活性。
While a number of oncogenes are expressed in a cell cycle-dependent manner, their role in the control of cell proliferation can only be established by a direct functional assay. The c-myc protein, upon microinjection into nuclei of quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma in the stimulation of cellular DNA synthesis. This suggested that c-myc protein, like platelet-derived growth factor (PDGF), may act as a competence factor in the cell cycle to promote the progression of cells to S phase. The presence in the medium of an antibody against PDGF abolished DNA synthesis induced by microinjected PDGF; however, the microinjected c-myc protein stimulated DNA synthesis even when its own antibody was present in the medium. The c-myc protein may act as an intracellular competence factor, while PDGF expresses its biological activity only from outside the cells.