OsRRM, a Spen-like rice gene expressed specifically in the endosperm
OsRRM, a Spen-like rice gene expressed specifically in the endosperm
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OsRRM，一种在胚乳中特异性表达的 Spen 样水稻基因
DOI：
10.1038/cr.2007.43
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发表时间：
2007-08-01
期刊：
影响因子：
44.1
通讯作者：
Cai, Xiu-Ling
中科院分区：
文献类型：
--
作者：
Chen, Shi-Yan;Wang, Zong-Yang;Cai, Xiu-Ling
我们利用启动子诱捕技术鉴定了一个水稻植株，命名为107（#），其中β-葡萄糖醛酸酶（GUS）报告基因在胚乳中特异表达。将T-DNA插入到植物基因组中，并通过插入鉴定出候选基因OsRRM。OsRRM启动子在水稻胚乳中特异性地指导GUS表达，类似于107（#）中观察到的GUS表达模式。OsRRM基因是水稻中的一个单拷贝基因，编码一个含有1005个氨基酸残基的核蛋白，具有两个RNA识别基序和一个Spen同源物和直向同源物C-末端结构域。Western blot分析证实OsRRM蛋白在水稻胚乳中特异表达。OsRRM在转基因植物中的异位表达导致了身材矮小、生长迟缓和结实率低等异常现象。结合已报道的Spen基因的功能，我们的数据表明OsRRM参与了水稻胚乳细胞发育的调控。
We used the promoter trap technique to identify a rice plant, named 107(#), in which the beta-glucuronidase (GUS) reporter gene was expressed specifically in the endosperm. A single copy of the T-DNA was inserted into the plant genome, and a candidate gene OsRRM was identified by the insertion. The OsRRM promoter directed GUS expression specifically in rice endosperm, analogous to the GUS expression pattern observed in 107(#). OsRRM is a single-copy gene in rice and encodes a nuclear protein containing 1005 amino-acid residues with two RNA recognition motifs and one Spen paralog and ortholog C-terminal domain. Western blot analysis confirmed that the OsRRM protein was specifically expressed in rice endosperm. Ectopic expression of OsRRM in transgenic plants led to abnormalities, such as short stature, retarded growth and low fructification rates. Our data, in conjunction with the reported function of Spen genes, implicated OsRRM in the regulation of cell development in rice endosperm.