Incresed glucose metabolism by FDG-PET correlates with reduced tumor angiogenesis in oral squamous cell carcinoma
Incresed glucose metabolism by FDG-PET correlates with reduced tumor angiogenesis in oral squamous cell carcinoma
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FDG-PET 增加的葡萄糖代谢与口腔鳞状细胞癌中肿瘤血管生成的减少相关
DOI：
10.1007/s10266-011-0024-3
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发表时间：
2012
期刊：
影响因子：
2.5
通讯作者：
Sano K.
中科院分区：
文献类型：
--
作者：
Nakamura M;Kitagawa Y;Yamazaki Y;Hata H;Kotsuji M;Fujibayashi Y;Okazawa H;Yonekura Y;Sano K.
脊髓小脑性共济失调7型（SCA 7）基因产物Ataxin-7（ATXN 7）定位于细胞核，并已显示作为无TATA结合蛋白的含TAF的-SPT 3-TAF 9-GCN 5-乙酰转移酶转录复合物的组分发挥作用，尽管也已检测到ATXN 7在人类SCA 7患者受影响神经元中的细胞质定位。在这里，我们定义了细胞质ATXN 7的生理功能。实时成像显示ATXN 7的细胞内分布动态变化，并且ATXN 7分布经常从细胞核转移到细胞质。免疫细胞化学和免疫沉淀表明，细胞质ATXN 7与微管（MT），和ATXN 7的表达稳定MT对诺考达唑治疗，而ATXN 7敲低增强MT降解。有趣的是，正常和突变体ATXN 7类似地与MT相关并同样稳定MT。总之，这些发现提供了ATXN 7在调节细胞骨架动力学中的新的生理功能，并表明异常的细胞骨架调节可能有助于SCA 7疾病病理学。
The spinocerebellar ataxia type 7 (SCA7) gene product, Ataxin-7 (ATXN7), localizes to the nucleus and has been shown to function as a component of the TATA-binding protein-free TAF-containing-SPT3-TAF9-GCN5-acetyltransferase transcription complex, although cytoplasmic localization of ATXN7 in affected neurons of human SCA7 patients has also been detected. Here, we define a physiological function for cytoplasmic ATXN7. Live imaging reveals that the intracellular distribution of ATXN7 dynamically changes and that ATXN7 distribution frequently shifts from the nucleus to the cytoplasm. Immunocytochemistry and immunoprecipitation demonstrate that cytoplasmic ATXN7 associates with microtubules (MTs), and expression of ATXN7 stabilizes MTs against nocodazole treatment, while ATXN7 knockdown enhances MT degradation. Interestingly, normal and mutant ATXN7 similarly associate with and equally stabilize MTs. Taken together, these findings provide a novel physiological function of ATXN7 in the regulation of cytoskeletal dynamics, and suggest that abnormal cytoskeletal regulation may contribute to SCA7 disease pathology.