THE BETA-1 DOMAIN OF THE MOUSE E-BETA CHAIN IS IMPORTANT FOR RESTRICTED ANTIGEN PRESENTATION TO HELPER T-CELL HYBRIDOMAS
THE BETA-1 DOMAIN OF THE MOUSE E-BETA CHAIN IS IMPORTANT FOR RESTRICTED ANTIGEN PRESENTATION TO HELPER T-CELL HYBRIDOMAS
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DOI：
10.1073/pnas.82.6.1678
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
--
作者：
FOLSOM, V;GAY, D;TONEGAWA, S
一种混合E β基因通过替换第2外显子构建。**图形 **。基因（其编码大部分β 1蛋白结构域）与来自.** 的相应外显子图形 **。基因将杂合基因导入d单倍型宿主淋巴瘤β-细胞系A20- 2 J和E α E β。由内源性二聚体组成。图形 **。链和杂交的产物。**图形 **。用免疫沉淀法从转染细胞的提取物中免疫沉淀基因。图形 **。单克隆抗体转染的细胞获得了呈递抗原的能力。**图形 **。辅助性T细胞杂交瘤，表明E β基因的第二外显子与E β基因的第二外显子相同。链编码II类限制性应答T细胞对抗原呈递细胞的限制性识别所需的序列。
A hybrid E.beta. gene was constructed by replacing the 2nd exon of the .**GRAPHIC**. gene (which encodes the majority of the .beta.1 protein domain) with the corresponding exon from the .**GRAPHIC**. gene. The hybrid gene was introduced into a d haplotype host, the lymphoma .beta.-cell line A20-2J, and an E.alpha.E.beta. dimer composed of the endogenous .**GRAPHIC**. chain and the product of the hybrid .**GRAPHIC**. gene was immunoprecipitated from extracts of transfected cells with an .**GRAPHIC**. monoclonal antibody. Transfected cells have acquired the ability to present antigen to .**GRAPHIC**. helper T-cell hybridomas, indicating that the 2nd exon of the gene for the E.beta. chain encodes sequences required for the restricted recognition of the antigen-presenting cell by the class II-restricted responder T-cell.