Stratum corneum chymotryptic enzyme in psoriasis
Stratum corneum chymotryptic enzyme in psoriasis
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DOI：
10.1007/s004030050393
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发表时间：
1999-04-01
影响因子：
3
通讯作者：
Egelrud, T
中科院分区：
文献类型：
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作者：
Ekholm, E;Egelrud, T
角质层糜蛋白酶(SCCE)是一种丝氨酸蛋白酶，可通过降解角质细胞间的细胞间黏附结构参与角质层的更新，也是一种潜在的细胞因子如白介素LP的表皮活化酶。本研究的目的是通过免疫组织化学方法研究SCCE在银屑病表皮中的表达，并通过生化分析阐明SCCE在银屑病鳞片中的性质。与正常皮肤相比，银屑病皮损中表达SCCE的细胞层数持续增加，而在非皮损的银屑病皮肤中，SCCE的表达模式发生了变化。在一些组织中，它与正常皮肤相似，而在另一些组织中，发现SCCE表达增加的证据。通过酶谱和免疫印迹，银屑病鳞片的提取物中既含有活性的SCCE，也含有酶失活的SCCE前体。此外，抑制物对凝胶排斥层析部分纯化后的提取物中发色酶底物活性的影响与具有酶活性的SCCE的存在是一致的。我们认为，SCCE在银屑病中的表达可能上调，并且在银屑病皮损中发生了无活性的SCCE前体向活性SCCE的转化。SCCE在银屑病病理生理学中的可能作用仍有待阐明，但应在未来的研究中加以考虑。
Stratum corneum chymotryptic enzyme (SCCE) is a serine protease which may function in the turnover of the stratum corneum by means of degradation of intercellular adhesive structures between corneocytes, It is also potentially an epidermal activating enzyme for cytokines such as interleukin-lp, The aim of this work was to study the expression of SCCE in psoriatic epidermis by means of immunohistochemistry, and to elucidate the nature of the SCCE present in psoriatic scales by means of biochemical analyses. In comparison to normal skin the number of cell layers expressing SCCE in psoriatic lesions was consistently increased, In nonlesional psoriatic skin the pattern of SCCE expression varied. It was similar to the pattern in normal skin in some biopsies, whereas in other biopsies evidence of an increased expression of SCCE was found, By means of zymography and immunoblotting, extracts of psoriatic scales were found to contain active SCCE as well as enzymatically inactive SCCE precursor. Also the effects of inhibitors on the activity towards a chromogenic protease substrate in the extracts after partial purification by gel exclusion chromatography were compatible with the presence of enzymatically active SCCE, We conclude that the expression of SCCE in psoriasis may be upregulated, and that the conversion of inactive SCCE-precursor to active SCCE occurs in the psoriatic lesion. The possible role of SCCE in the pathophysiology of psoriasis remains to be elucidated, but should be considered in future studies.