Divergent effects of propranolol on neutrophil superoxide release: involvement of phosphatidic acid and diacylglycerol as second messengers.
Divergent effects of propranolol on neutrophil superoxide release: involvement of phosphatidic acid and diacylglycerol as second messengers.
复制标题
普萘洛尔对中性粒细胞超氧化物释放的不同影响：磷脂酸和二酰甘油作为第二信使的参与。
DOI：
10.1016/0006-291x(91)91581-v
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发表时间：
1991
影响因子：
3.1
通讯作者：
Taylor,GS
中科院分区：
文献类型：
--
作者：
English,D;Taylor,GS
相对高水平的普萘洛尔（170 μM）显著减弱了用FMLP加细胞松弛素B或20.0 mM NaF刺激的中性粒细胞中1，2-二酰甘油的生成。这种效果是由于磷脂酸磷酸水解酶的抑制，因为它伴随着刺激细胞的有机提取物中磷脂酸的回收率相应增加。虽然普萘洛尔增强与FMLP单独治疗的中性粒细胞中的磷脂酸水平，药物只有轻微的抑制作用，在这些细胞中的甘油二酯的产生。普萘洛尔对单独用FMLP处理的中性粒细胞中PA水平增强的作用与FMLP诱导的O2 −生成增强密切相关。然而，普萘洛尔在用FMLP+细胞松弛素B或20.0 mM NaF刺激的中性粒细胞中诱导了类似的O2 −生成的剂量依赖性抑制。这些结果与磷脂酸和甘油二酯是中性粒细胞O2 −释放最佳起始所需的假设一致。
Relatively high levels of propranolol (170 μM) markedly attenuated the generation of 1, 2 diacylglycerol in neutrophils stimulated with either FMLP plus cytochalasin B or with 20.0 mM NaF. This effect resulted from inhibition of phosphatidic acid phosphohydrolase as it was accompanied by a corresponding increase in the recovery of phosphatidic acid in organic extracts of stimulated cells. Although propranolol enhanced phosphatidic acid levels in neutrophils treated with FMLP alone, the drug had only a slight inhibitory influence on diglyceride generation in these cells. The effect of propranolol on enhancement of PA levels in neutrophils treated with FMLP alone strongly correlated with enhancement of FMLP-induced O 2− generation. However, propranolol induced a similar dose-dependent inhibition of O 2− generation in neutrophils stimulated with either FMLP+ cytochalasin B or with 20.0 mM NaF. These results are consistent with the hypothesis that both phosphatidic acid and diacylglycerol are required for optimal initiation of neutrophil O 2− release.