Two critical periods of protein and glycoprotein synthesis in memory consolidation for visual categorization learning in chicks
Two critical periods of protein and glycoprotein synthesis in memory consolidation for visual categorization learning in chicks
复制标题
DOI：
10.1101/lm.4.5.401
复制
发表时间：
1998-01-01
影响因子：
2
通讯作者：
Rose, SPR
中科院分区：
文献类型：
--
作者：
Tiunova, AA;Anokhin, KV;Rose, SPR
用蛋白质合成抑制剂茴香霉素（ANI）和糖蛋白合成抑制剂2-脱氧半乳糖（2-D-gal）研究了2日龄雏鸡视觉分类训练后记忆巩固的情况。ANI（0.6 μ mol/鸡）和2-D-gal（40 μ mol/鸡）在训练前1小时至训练后23小时的不同时间间隔脑内注射。在训练后24小时测试保持力。ANI和2-D-gal注射揭示了两个时期的记忆敏感性药物干预。ANI受损保留时，从5分钟前到30分钟后的培训或从4小时至5小时后的培训，从而表明，巩固长期记忆在这个任务需要两个时期的蛋白质合成。2-D-Gal在训练前5 min至训练后5 min注射时首先产生记忆缺失。训练后5分钟至5小时之间注射对保持没有影响。2-D-半乳糖引起的第二个记忆损害期从训练后5小时开始，持续到训练后21小时。在训练后23小时给予2-D-gal，在24小时或26小时的测试中不影响保持。这些结果与蛋白质和糖蛋白合成的两个波对于长期记忆的形成是必要的这一假设是一致的。在本任务中，2-D-半乳糖导致的表现障碍的持续时间延长可能反映了分类学习形式的记忆巩固期延长。
A protein synthesis inhibitor, anisomycin (ANI), and an inhibitor of glycoprotein synthesis, 2-deoxygalactose (2-D-gal), were used to investigate memory consolidation following visual categorization training in 2-day-old chicks. ANI (0.6 mu mole/chick) and 2-D-gal (40 mu moles/chick) were injected intracerebrally at different time intervals from 1 hr before to 23 hr after the training. Retention was tested 24 hr post-training. Both ANI and 2-D-gal injections revealed two periods of memory sensitivity to pharmacological intervention. ANI impaired retention when injected from 5 min before to 30 min after the training or from 4 hr to 5 hr post-training, thus demonstrating that consolidation of long-term memory in this task requires two periods of protein synthesis. 2-D-Gal first produced an amnesia when it was injected in the interval from 5 min before to 5 min after the training. Injections made between 5 min and 5 hr post-training were without effect on the retention. The second period of memory impairment by 2-D-gal started at 5 hr post-training and lasted until 21 hr after the training. Administration of 2-D-gal made 23 hr after the training did not influence retention in the test at either 24 hr or 26 hr. These results are consistent with the hypothesis that two waves of protein and glycoprotein synthesis are necessary for the formation of long-term memory. The prolonged duration of performance impairment by 2-D-gal in the present task might reflect an extended memory consolidation period for a categorization form of learning.