REGULATION OF GLYCOGEN IN THE DENTATE GYRUS OF THE INVITRO GUINEA-PIG HIPPOCAMPUS - EFFECT OF COMBINED DEPRIVATION OF GLUCOSE AND OXYGEN
REGULATION OF GLYCOGEN IN THE DENTATE GYRUS OF THE INVITRO GUINEA-PIG HIPPOCAMPUS - EFFECT OF COMBINED DEPRIVATION OF GLUCOSE AND OXYGEN
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DOI：
10.1016/0165-0270(89)90019-8
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发表时间：
1989-05-01
影响因子：
3
通讯作者：
LIPTON, P
中科院分区：
文献类型：
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作者：
LIPTON, P
哺乳动物的脑糖原足以支持几分钟的氧化代谢。本研究主要是建立豚鼠海马切片作为研究该糖原功能和调控的模型。在36°C孵育的第一个小时内，切片糖原下降到6 nmol/mg干wt.，但在接下来的3小时内恢复到20 nmol/mg干wt.，与原位值相似。齿状回分子层糖原浓度是整个海马切片糖原浓度的两倍，提示其分布与代谢需求有关。当从培养基中去除葡萄糖和氧气时，糖原和ATP在6分钟内下降到50%。糖原下降不受长期钙消耗或3-异丁基- 1-甲基黄嘌呤（一种腺苷拮抗剂）的影响。通过用肌酸预孵育切片，它可以明显减缓，这也减缓了ATP的下降。在体外缺血模型中，ATP分解和随后的5′-AMP升高激活了糖原动员。
Mammalian brain glycogen is adequate to support oxidative metabolism for several minutes. The present studies were done primarily to develop the guinea pig hippocampal slice as a model for studying the function and regulation of that glycogen.Slice glycogen falls to 6 nmol/mg dry wt. during the first hour of incubation at 36°C but during the next 3 h recovers to 20 nmol/mg dry wt., similar to in situ values. Glycogen concentration in the dentate gyrus molecular layer is double its value in the whole hippocampal slice, suggesting its distribution is related to metabolic demand. When both glucose and oxygen are removed from the medium, glycogen and ATP fall to 50% within 6 min. The glycogen fall is unaffected by prolonged calcium depletion or by 3-isobutyl 1-methylxanthine, an adenosine antagonist. It is markedly slowed by preincubating the slice with creatine, which also slows the fall in ATP. It is concluded that ATP breakdown and subsequent increased 5′-AMP is activating glycogen mobilization in this in vitro model of ischemia.