Joint unloading inhibits articular cartilage degeneration in knee joints of a monosodium iodoacetate-induced rat model of osteoarthritis
Joint unloading inhibits articular cartilage degeneration in knee joints of a monosodium iodoacetate-induced rat model of osteoarthritis
复制标题
关节卸荷抑制碘乙酸钠诱导的骨关节炎大鼠膝关节关节软骨退变
DOI：
10.1016/j.joca.2019.03.001
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发表时间：
2019-07-01
影响因子：
7
通讯作者：
Hoso, M.
中科院分区：
文献类型：
--
作者：
Takahashi, I.;Matsuzaki, T.;Hoso, M.
目的：本研究的目的是探讨机械卸载如何影响髌股关节（PF）和胫股关节（TF）关节的碘乙酸（MIA）诱导的大鼠骨关节炎（OA）模型的关节软骨退变。设计：本研究涉及60只雄性大鼠。通过向双膝关节内注射MIA来诱导OA。所有动物均分为两组：久坐（SE）和后肢去负荷（HU）组。在PF和TF关节的关节软骨的组织病理学变化进行了评价，使用骨关节炎研究学会国际（OARSI）评分和改良的Mankin评分在2和4周后MIA injection.Results：在SE和HU组，在PF和TF关节OA的代表性组织病理学变化检测。SE和HU组注射0.2 mg或1.0 mg MIA后，PF和TF关节的OARSI和改良Mankin评分有随时间推移而增加的趋势。HU组的OARSI评分显著低于SE组[P < 0.0001（4周时注射1.0 mg）;改良Mankin评分：P = 0.0116（4周时注射0.2 mg）; P = 0.0004且< 0.0001（第2周和第4周分别注射1.0 mg）]。这项研究揭示了新的组织学证据，表明卸载条件抑制关节软骨退变，并在许多领域的基础和临床研究涉及OA是有益的。(C)2019年，任作家。由Elsevier Ltd代表国际骨关节炎研究协会出版。
Objective: The aim of the study was to examine how mechanical unloading affects articular cartilage degeneration in the patellofemoral (PF) and tibiofemoral (TF) joints of a monosodium iodoacetate (MIA)-induced rat model of osteoarthritis (OA).Design: The study involved 60 male rats. OA was induced by intra-articular injecting MIA into both knee joints. All animals were equally divided into two groups: sedentary (SE) and hindlimb unloading (HU) groups. Histopathological changes in the articular cartilage of the PF and TF joints were evaluated using the Osteoarthritis Research Society International (OARSI) score and modified Mankin score at 2 and 4 weeks after MIA injection.Results: In the SE and HU groups, representative histopathological changes in OA were detected in the PF and TF joints. The OARSI and modified Mankin scores for the PF and TF joints tended to increase over time after the injection of 0.2 mg or 1.0 mg of MIA in the SE and HU groups. Both the scores for the HU group were significantly lower than those for the SE group [OARSI score: P < 0.0001 (1.0-mg injection at 4 weeks); modified Mankin score: P = 0.0116 (0.2-mg injection at 4 weeks); P = 0.0004 and < 0.0001 (1.0-mg injection at 2 and 4 weeks, respectively)].Conclusion: This study revealed new histological evidence that indicates that unloading condition suppresses articular cartilage degeneration and is beneficial in many areas of basal and clinical research involving OA. (C) 2019 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.