Fecal Microbial Community Structure Is Stable over Time and Related to Variation in Macronutrient and Micronutrient Intakes in Lactating Women
Fecal Microbial Community Structure Is Stable over Time and Related to Variation in Macronutrient and Micronutrient Intakes in Lactating Women
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DOI：
10.3945/jn.115.211110
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发表时间：
2015-10-01
影响因子：
4.2
通讯作者：
McGuire, Michelle K.
中科院分区：
文献类型：
--
作者：
Carrothers, Janae M.;York, Mara A.;McGuire, Michelle K.
背景：某些成年人群的粪便微生物群已得到表征，但对其哺乳期间的群落结构知之甚少。目的：我们对哺乳期间的母体粪便微生物群进行了表征，并探讨了营养等可能的调节因素。方法：从产后 2 天至 6 个月收集了 20 名哺乳期妇女的粪便样本，并利用高通量测序对细菌分类群进行了表征。使用堆积条形图、主成分分析和多变量分析（例如非度量多维标度和典型相关分析）对细菌群落结构（在每个分类水平）以及细菌类群与环境和饮食变量之间的关系进行可视化和分析。结果：复杂的细菌群落结构与之前针对其他成年人群发表的结果有些相似（尽管存在一些显着差异），并且与产后时间或人体测量或环境变量没有明显的关联。然而，Spearman 等级相关性表明，泛酸、核黄素、维生素 B-6 和维生素 B-12 摄入量的增加与普氏菌相对丰度的增加有关（r 分别 = 0.45、0.39、0 34 和 0.24；P
Background: The fecal microbiota has been c'haracterized in some adult populations, but little is known about its community structure during lactation.Objectives: We characterized the maternal fecal microbiome during lactation-and explored possible mediating factors such as nutrition.Methods: Fecal samples were collected from 20 lactating women from 2 d to 6 mo postpartum, and bacterial taxa were characterized with the use of high-throughput sequencing. Bacterial community structure (at each taxonomic level) and relations between bacterial taxa and environmental and dietary variables were visualized and analyzed with the use of stacked bar charts, principal component analysis, and-multivariate analyses such as nonmetric multidimensional scaling and canonical correlation analysis.Results: Complex bacterial community structure was somewhat similar to those previously published for other adult populations (although there were some notable differences), and there were no clear associations with time postpartum or anthropometric or environmental variables. However, Spearman rank correlations suggested that increased intake of pantothenic acid, riboflavin, vitamin B-6,-and vitamin-B-12 were related to increased relative abundance of Prevotella (r= 0.45, 0.39, 0 34, and 0.24, respectively; P