Circulating level of microRNA-126 may be a potential biomarker for recovery from smoking-related vascular damage in middle-aged habitual smokers.
Circulating level of microRNA-126 may be a potential biomarker for recovery from smoking-related vascular damage in middle-aged habitual smokers.
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DOI：
10.1016/j.ijcha.2015.02.012
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发表时间：
2015-06-01
期刊：
影响因子：
--
通讯作者：
Fujii S
中科院分区：
文献类型：
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作者：
Sugiura T;Dohi Y;Yamashita S;Iwaki S;Ito S;Sanagawa A;Ohte N;Fujii S
吸烟会促进血管内皮损伤并加速动脉粥样硬化的进展。本研究的目的是检查富含血管内皮的 microRNA-126 (miR-126) 的循环水平是否可以作为吸烟相关血管损伤恢复的新型生物标志物，miR-126 被强调为基因表达的调节因子。中年男性吸烟者 (n = 30) 被纳入研究并被指导戒烟。在戒烟 8 周之前和之后对他们的临床特征和实验室检查结果（包括 miR-126 的表达）进行了调查。测量可替宁（尼古丁的代谢物）的血清水平以确认戒烟。评估外周小血管的内皮功能并表示为反应性充血外周动脉张力测定（RH-PAT）指数。通过实时定量PCR分析血浆中miR-126的表达。基线时，血清可替宁水平与 RH-PAT 指数呈负相关（r=0.48，P<0.01），与非 HDL 胆固醇（r=0.53，P<0.01）和 HOMA-IR（r=0.52，P<0.01）等代谢参数水平呈正相关。 8 周后，所有受试者的 RH-PAT 指数没有显着变化，因为只有 13 名受试者能够戒烟。然而，RH-PAT 指数的变化与收缩压的变化具有显着相关性（r = − 0.54，P < 0.01）。在完全戒烟的吸烟者中（n = 13），RH-PAT 指数和血浆 miR-126 水平显着升高（分别 P < 0.05）。戒烟 8 周后，内皮损伤得到改善，循环 miR-126 的血浆水平增加。这些发现表明 miR-126 可能用作吸烟引起的血管损伤恢复的生物标志物。吸烟会促进血管内皮损伤。中年男性吸烟者被纳入并被指导戒烟。研究人员对他们的临床特征和 miR-126 循环水平进行了研究。 8 周后，内皮功能和循环 miR-126 恢复。循环中的 miR-126 可能是吸烟相关血管损伤的生物标志物。
Cigarette smoking promotes vascular endothelial damage and accelerates progression of atherosclerosis. The purpose of this study was to examine whether the circulating level of vascular endothelium-enriched microRNA-126 (miR-126), which is highlighted as a regulator of gene expression, would serve as a novel biomarker for recovery from smoking-related vascular damage. Middle-aged male smokers (n = 30) were enrolled and instructed to stop smoking. Their clinical profiles and laboratory findings including expression of miR-126 were investigated before and after 8 weeks of smoking cessation. Serum levels of cotinine, metabolites of nicotine, were measured to confirm smoking cessation. Endothelial function for peripheral small vessels was assessed and expressed as reactive hyperemia peripheral arterial tonometry (RH-PAT) index. The expression of miR-126 in plasma was analyzed by quantitative real-time PCR. At baseline, serum cotinine levels were inversely correlated with RH-PAT index (r = − 0.48, P < 0.01) and positively correlated with levels of metabolic parameters such as non-HDL cholesterol (r = 0.53, P < 0.01) and HOMA-IR (r = 0.52, P < 0.01). The RH-PAT index was not significantly changed after 8 weeks in all subjects, because only 13 subjects could attain smoking cessation. However, changes in the RH-PAT index showed a significant correlation with those in systolic blood pressure (r = − 0.54, P < 0.01). In smokers who completely attained smoking cessation (n = 13), RH-PAT index and plasma levels of miR-126 were significantly increased (P < 0.05, respectively). Endothelial damage was improved and plasma levels of circulating miR-126 were increased after 8 weeks of smoking cessation. These findings suggested a potential use of miR-126 as a biomarker for recovery from smoking-induced vascular damage. Cigarette smoking promotes vascular endothelial damage. Middle-aged male smokers were enrolled and instructed to stop smoking. Their clinical profiles and circulating levels of miR-126 were investigated. Endothelial function and circulating miR-126 were recovered after 8 weeks. The circulating miR-126 may be a biomarker for smoking-related vascular damage.