Detection of HIV in bone allografts prepared from AIDS autopsy tissue.
Detection of HIV in bone allografts prepared from AIDS autopsy tissue.
复制标题
从艾滋病尸检组织制备的同种异体骨移植物中检测艾滋病毒。
DOI：
10.1097/00003086-199307000-00050
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发表时间：
1993
影响因子：
4.2
通讯作者：
Robert Oleary
中科院分区：
文献类型：
--
作者：
NORMAN P. Salzman;Miltiades Psallidopoulos;ANNAMARIE B. Prewett;Robert Oleary
对来自艾滋病患者的经处理的骨进行了检测，以确定是否存在人类免疫缺陷病毒1型（HIV-1）。用于处理骨同种异体移植物的初步程序包括去除外源性材料和两个冻融循环。虽然在尸检时采集的血浆和骨髓细胞中很容易观察到感染性病毒，但在处理的骨碎片中未检测到感染性病毒。然而，通过使用聚合酶链反应程序，前病毒的HIV DNA的存在下，可以证明在处理骨同种异体移植物从这个供体。虽然防止HIV通过同种异体移植物传播的最佳保障是严格排除血清阳性个体作为供体的标准，但是在收集和处理来自受感染供体的组织的情况下，处理骨同种异体移植物的适当程序可以进一步降低HIV通过骨同种异体移植物传播的可能性。
Processed bone from an AIDS patient was tested for the presence of the human immunodeficiency virus type 1 (HIV-1). The preliminary procedures used to process bone allografts included removal of adventitious material and two cycles of freeze thawing. Although infectious virus was readily observed in plasma and bone marrow cells taken at autopsy, no infectious virus was detected in processed bone fragments. However, by using the polymerase chain reaction procedure, the presence of proviral HIV DNA could be demonstrated in processed bone allografts from this donor. Whereas the best safeguard against transmission of HIV by allografts is rigorous criteria for the exclusion of seropositive individuals as donors, proper procedures for processing bone allografts can further reduce the possibility of HIV transmission by bone allografts in cases where tissue from an infected donor is collected and processed.