Timing of completion of the first meiotic division in bovine oocytes after maintenance of meiotic arrest with cycloheximide and their subsequent development.
Timing of completion of the first meiotic division in bovine oocytes after maintenance of meiotic arrest with cycloheximide and their subsequent development.
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用放线菌酮维持减数分裂停滞后牛卵母细胞第一次减数分裂的完成时间及其随后的发育。
DOI：
10.1292/jvms.60.523
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
A. Iritani
中科院分区：
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作者：
K. Saeki;Y. Nagao;M. Kishi;M. Nagai;A. Iritani
本研究考察了被环己亚胺（CHX）抑制的牛卵母细胞减数分裂I期完成的时间，并确定了随后受精和发育的最佳成熟间隔。大多数经CHX处理的卵母细胞在成熟培养中16小时达到中期II，而对照卵母细胞在20小时达到中期II。经chx处理16小时的卵母细胞能正常受精，但不能发育成囊胚。培养20小时的成熟与对照卵母细胞的发育相当。结果表明，chx处理的卵母细胞比对照的卵母细胞提前4小时完成核成熟，但与对照卵母细胞的成熟间隔相同，后续发育为囊胚。
This study examined the timing of completion of meiosis I of bovine oocytes in which meiotic resumption had been inhibited by cycloheximide (CHX), and also determined the optimum interval of maturation in culture for subsequent fertilization and development. Most oocytes treated with CHX reached metaphase II at 16 hr in the maturation culture, while control oocytes did at 20 hr. CHX-treated oocytes cultured for 16 hr were normally fertilized but failed to develop into blastocysts. Maturation in culture for 20 hr resulted in comparable development for control oocytes. The results indicate that nuclear maturation of CHX-treated oocytes was completed 4 hr faster than for control oocytes, however the same interval of maturation as that of control oocytes is necessary for subsequent development to blastocysts.
CHIAN, R. C.：“老化牛卵母细胞在培养物中成熟后的体外受精和早期分裂”动物发生学。
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基质金属蛋白酶11基因在柴山羊胎盘和子宫中的表达
DOI：
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发表时间：
1995
期刊：
影响因子：
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作者：
Takahito Hara;Satoshi Tanaka;Hiroshi Sato;M. Seiki;H. Koi;A. Suenaga;H. Tojo;C. Tachi
通讯作者：
C. Tachi