New phosphoglucose isomerase mutants of Escherichia coli
New phosphoglucose isomerase mutants of Escherichia coli
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大肠杆菌新的磷酸葡萄糖异构酶突变体
DOI：
10.1128/jb.122.3.1172-1174.1975
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发表时间：
1975
影响因子：
3.2
通讯作者：
D. Fraenkel
中科院分区：
文献类型：
--
作者：
R. Vinopal;J. Hillman;H. Schulman;W. Reznikoff;D. Fraenkel
用来证明磷酸葡萄糖异构酶和葡萄糖本身不是大肠杆菌的必要成分的突变体除了通过作图之外，还没有从遗传上进行表征。我们现在描述两个新的PGI突变体，一个是琥珀，另一个是Mu噬菌体插入，推测这两个突变都是完全失活的突变。新的突变没有给出与前面描述的有明显不同的表型。然而，它们可能是某些生理研究的首选，我们为此准备了一个新的双突变株DF214，它在PGI中插入了Mu，在zwf(6-磷酸岩藻糖脱氢酶)中缺失。
The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and the other a Mu-phage insertion, presumably both complete inactivation mutations. The new mutations do not give a phenotype markedly different from those described earlier. However, they might be preferred for certain physiological studies, and we have prepared for this a new double mutant, strain DF214, with a Mu insertion in pgi and a deletion in zwf (flucose 6-phosphate dehydrogenase).