Evaluation of antibodies against feline coronavirus 7b protein for diagnosis of feline infectious peritonitis in cats
Evaluation of antibodies against feline coronavirus 7b protein for diagnosis of feline infectious peritonitis in cats
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DOI：
10.2460/ajvr.69.9.1179
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发表时间：
2008-09-01
影响因子：
1
通讯作者：
Kania, Stephen A.
中科院分区：
文献类型：
--
作者：
Kennedy, Melissa A.;Abd-Eldaim, Mohamed;Kania, Stephen A.
目的：探讨猫冠状病毒（FCoV） 7b蛋白的表达是否与猫感染性腹膜炎（FIP）的发生一致。样本群体：95份血清样本用于各种诊断分析，20份来自无特定病原体猫的样本作为阴性对照样本进行检测。将FCoV毒株的7b基因克隆到蛋白表达载体中。合成的重组蛋白被生产出来，并通过血清样品的免疫印迹分析用于抗体检测。结果与fcov特异性抗体免疫荧光法（IFA）比较，并与健康状况相关。结果：健康fa血清阴性猫血清抗7b蛋白抗体均为阴性。一些通过IFA检测到fcov特异性抗体的健康猫血清抗7b蛋白抗体呈阴性。FIP猫的血清中含有抗7b蛋白的抗体，包括常规FA检测结果为阴性的猫。然而，一些健康猫，以及患有FIP以外疾病的猫，通过IFA对FCoV呈血清阳性，也对7b蛋白呈血清阳性。结论与临床相关性：在大多数fcov感染的猫中，检测到7b蛋白的抗体，表明该蛋白的表达。该蛋白的血清阳性对FCoV毒性生物型或FIP诊断不具有特异性
Objective-To determine whether expression of feline coronavirus (FCoV) 7b protein, as indicated by the presence of specific serum antibodies, consistently correlated with occurrence of feline infectious peritonitis (FIP) in cats.Sample Population-95 serum samples submitted for various diagnostic assays and 20 samples from specific-pathogen-free cats tested as negative control samples.Procedures-The 7b gene from a virulent strain of FCoV was cloned into a protein expression vector. The resultant recombinant protein was produced and used in antibody detection assays via western blot analysis of serum samples.Results were compared with those of an immunofluorescence assay (IFA) for FCoV-specific antibody and correlated with health status. Results-Healthy FA-seronegative cats were seronegative for antibodies against the 7b protein. Some healthy cats with detectable FCoV-specific antibodies as determined via IFA were seronegative for antibodies against the 7b protein. Serum from cats with FIP had antibodies against the 7b protein, including cats with negative results via conventional FA. However, some healthy cats, as well as cats with conditions other than FIP that were seropositive to FCoV via IFA, were also seropositive for the 7b protein.Conclusions and Clinical Relevance-Expression of the 7b protein, as indicated by detection of antibodies against the protein, was found in most FCoV-infected cats. Seropositivity for this protein was not specific for the FCoV virulent biotype or a diagnosis of FIP