Optimized methodology for the simultaneous extraction of glucosinolates, phenolic compounds and antioxidant capacity from maca (Lepidium meyenii)
Optimized methodology for the simultaneous extraction of glucosinolates, phenolic compounds and antioxidant capacity from maca (Lepidium meyenii)
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DOI：
10.1016/j.indcrop.2013.06.021
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发表时间：
2013-08-01
影响因子：
5.9
通讯作者：
Pedreschi, Romina
中科院分区：
文献类型：
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作者：
Campos, David;Chirinos, Rosana;Pedreschi, Romina
玛卡是一种备受赞赏的安第斯作物，由于其各种生物活性化合物，具有多种归因于健康的声称。采用响应面法优化玛咖下胚轴硫代葡萄糖苷、总酚和抗氧化能力的提取工艺。采用基于响应面法（RSM）的Box-Behnken设计（BBD）研究了不同提取参数（温度（℃）、液固比（ml/g）、时间（min）和乙醇浓度（%））对GL、TPC和AC提取率的影响。在47、70和70 ℃，液固比为10、16和10 ml/g，提取时间为90、30和48 min，乙醇浓度为60%的条件下，得到最大提取率分别为16.4 μ mol/g干物质（DM），9.89 mg没食子酸当量（GAE）/g DM和61.4 μ mol trolox当量（TE）/g。对GL、TPC和AC分别为60%和50%。响应面法（RSM）确定了同时提取GLS、TPC和AC的最佳提取条件为70 ℃、10 ml/g、90 min和58%乙醇，GLS、TPC和AC的提取率分别为14.2 μ mol/g DM、9.3 mg GAE/g DM和56.9 μ mol TE/g。在这些条件下，glucotropaeolin占总GL的79%，儿茶素和没食子儿茶素衍生物占总酚的97%。优化的条件可以成功地用于营养品行业从玛咖粉中提取生物活性化合物。(C)2013爱思唯尔有限公司版权所有。
Maca is a highly appreciated Andean crop with multiple attributed health claims due to its assortment of bioactive compounds. The extraction parameters of glucosinolates (GLs), total phenolic compounds (TPC) and antioxidant capacity (AC) of maca (Lepidium meyenii) hypocotyls were optimized using response surface methodology (RSM). The effect of different extraction parameters: temperature (degrees C), liquid-to-solid ratio (ml/g), time (min) and ethanol concentration (%) on GLs, TPC and AC extraction yields were investigated using a Box-Behnken design (BBD) based on response surface methodology (RSM). Maximum yields of 16.4 mu mol/g of dried matter (DM), 9.89 mg of gallic acid equivalents (GAE)/g of DM and 61.4 mu mol of trolox equivalents (TE)/g were obtained at conditions of 47, 70 and 70 degrees C, liquid-to-solid ratio of 10,16 and 10 ml/g, extraction time of 90,30 and 48 min and ethanol concentration of 60%, 60% and 50%, for GLs, TPC and AC respectively. Optimal extraction conditions for the simultaneous extraction of GLs, TPC and AC determined by RSM were 70 degrees C, 10 ml/g, 90 min and 58% ethanol with extraction yields of 14.2 mu mol/g of DM, 9.3 mg of GAE/g of DM and 56.9 mu mol TE/g for GLs, TPC and AC respectively. Under these conditions, glucotropaeolin represented 79% of total GLs and catechins and gallocatechin derivatives represented 97% of total phenolics. The optimized conditions could be successfully employed by the Nutraceutical industry to extract bioactive compounds from maca flour. (C) 2013 Elsevier B.V. All rights reserved.