HEMOLYTIC-ACTIVITY OF A CYCLIC PEPTIDE RO09-0198 ISOLATED FROM STREPTOVERTICILLIUM
HEMOLYTIC-ACTIVITY OF A CYCLIC PEPTIDE RO09-0198 ISOLATED FROM STREPTOVERTICILLIUM
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DOI：
10.1016/0005-2736(88)90192-7
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发表时间：
1988-05-24
期刊：
影响因子：
--
通讯作者：
INOUE, K
中科院分区：
文献类型：
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作者：
CHOUNG, SY;KOBAYASHI, T;INOUE, K
Ro 09 -0198是从灰轮枝链霉菌培养物中分离的一种环肽，可诱导红细胞溶解。用磷脂酰乙醇胺预孵育的肽降低了溶血活性，而存在于红细胞中的其他磷脂在自然界中没有影响。对与肽相互作用所必需的磷脂酰乙醇胺的结构要求的研究表明，Ro 09 -0198严格识别磷脂酰乙醇胺的特定化学结构：二烷基磷酸乙醇胺以及1-酰基甘油磷酸乙醇胺对Ro 09 -0198诱导的溶血显示出与二酰基磷脂酰乙醇胺相同的抑制作用，而磷酸乙醇胺没有抑制作用。磷脂酰-N-单甲基乙醇胺和烷基磷酸丙醇胺均无抑制作用。因此，疏水链是相互作用所必需的，并且磷酸乙醇胺部分被肽精确识别。 Ro-09-0198诱导的溶血具有温度依赖性，不同动物种属间溶血的敏感性差异很大。
Ro09-0198, a cyclic peptide isolated from culture filtrates of Streptoverticillium griseoverticillatum, induced lysis of erythrocytes. Preincubation of the peptide with phosphatidylethanolamine reduced the hemolytic activity, whereas other phospholipids present in erythrocytes in nature had no effect. A study of the structural requirements on phosphatidylethanolamine necessary for interaction with the peptide indicates that Ro09-0198 recognizes strictly a particular chemical structure of phosphatidylethanolamine: dialkylphosphoethanolamine as well as 1-acylglycerophosphoethanolamine showed the same inhibitory effect on hemolysis induced by Ro09-0198 as diacylphosphatidylethanolamine, whereas phosphoethanolamine gave no inhibitory effect. Neither phosphatidyl-N-monomethylethanolamine nor alkylphosphopropanolamine had an inhibitory effect. Consequently, the hydrophobic chain is necessary for the interaction and the phosphoethanolamine moiety is exactly recognized by the peptide. Ro-09-0198-induced hemolysis was temperature-dependent and the sensitivity of hemolysis differed greatly among animal species.