Development and comparison of immunochromatographic strips with three nanomaterial labels: Colloidal gold, nanogold-polyaniline-nanogold microspheres (GPGs) and colloidal carbon for visual detection of salbutamol
Development and comparison of immunochromatographic strips with three nanomaterial labels: Colloidal gold, nanogold-polyaniline-nanogold microspheres (GPGs) and colloidal carbon for visual detection of salbutamol
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三种纳米材料标记的免疫层析试纸条的研制及比较：胶体金、纳米金 - 聚苯胺 - 纳米金微球（GPGs）和胶体碳用于沙丁胺醇的可视化检测
DOI：
10.1016/j.bios.2016.05.032
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发表时间：
2016-11-15
影响因子：
12.6
通讯作者：
Wang, Shuo
中科院分区：
文献类型：
--
作者：
Liu, Bing;Wang, Lingling;Wang, Shuo
本研究分别用沙丁胺醇（SAL）抗体标记了胶体金、纳米金-聚苯胺-纳米金微球（GPGs）和胶体碳三种纳米材料。我们的目的是开发带有这些纳米材料标记的免疫层析试纸条，并确定其在视觉检测SAL中的性能。对于胶体金基试纸条，标准溶液中SAL的检出限为1.0μgL-1，肉类样品中SAL的检出限为5.0μgkg(-1)。对于GPG基和胶体碳基试纸条，标准溶液中的检测限为2.0μgL-1，肉样品中的检测限为10μgkg(-1)。使用测试条获得的结果与使用商业试剂盒获得的结果高度一致，表明这些测试条的准确性很高。还发现这三个试纸条在实验室条件下可稳定保存长达 18 周。就灵敏度而言，胶体金基试纸条略优于其他两者。对于GPG基试纸条和胶体碳基试纸条，不同批次获得的结果差异很小（一致性高），并且稳定性比胶体金基试纸条好得多。我们的结果表明，胶体碳可以用作免疫层析测试中的标记；它还可以帮助降低成本并扩大生产规模。使用胶体碳标记的免疫层析测试条可以成为现场应用中 SAL 测定的一种快速且廉价的方法。 (C) 2016 Elsevier B.V. 保留所有权利。
In this study, the three nanomaterials: colloidal gold, nanogold-polyaniline-nanogold microspheres (GPGs) and colloidal carbon were respectively labeled with the antibody against salbutamol (SAL). We aimed to develop immunochromatographic strips with these nanomaterial labels and determine their performance in visual detection of SAL For the colloidal gold-based strip, the detection limit of SAL was 1.0 mu g L-1 in standard solution and 5.0 mu g kg(-1) in meat samples. For the GPG- and colloidal carbon-based strips, the limit of detection was 2.0 mu g L-1 in standard solution and 10 mu g kg(-1) in meat samples. The results obtained using the test strips were found to be highly consistent with those obtained using a commercial kit, indicating the high accuracy of these strips. The three strips were also found to be stable up to 18 weeks under laboratory conditions. In terms of sensitivity, the colloidal gold-based strip was slightly better than the other two. For the GPG- and colloidal carbon-based strips, the difference between the results obtained for different batches was small (high consistency), and the stability was much better than that of the colloidal gold-based one. Our results indicate that colloidal carbon can be used as a label in immunochromatographic tests; it can also help reduce the cost involved and scale-up the production. The use of immunochromatographic test strips labeled with colloidal carbon can be a rapid and inexpensive method for SAL assays in on-site applications. (C) 2016 Elsevier B.V. All rights reserved.