AUTORADIOGRAPHIC ANALYSIS OF [H-3] KETANSERIN BINDING IN THE HUMAN BRAIN POSTMORTEM - EFFECT OF SUICIDE
AUTORADIOGRAPHIC ANALYSIS OF [H-3] KETANSERIN BINDING IN THE HUMAN BRAIN POSTMORTEM - EFFECT OF SUICIDE
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DOI：
10.1016/0006-8993(90)90274-f
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发表时间：
1990-01-22
期刊：
影响因子：
2.9
通讯作者：
BIEGON, A
中科院分区：
文献类型：
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作者：
GROSSISSEROFF, R;SALAMA, D;BIEGON, A
以[3H]酮色林为配体，对24具人死后脑组织进行5-HT2受体的体外定量放射自显影。自杀者捐赠了12个大脑，对照组捐赠了12个。我们发现，在对照组的几个大脑区域中，5-HT2受体随着年龄的增长出现了特征性的下降。这种年龄依赖性的酮色胺结合在自杀组的某些脑区不存在。我们还发现，与年龄匹配的对照组相比，年轻自杀组中酮色胺结合位点的密度显著减少，但在解剖学上是选择性的。这种减少在部分前额皮质中很明显。对大量自杀者（n = 20）和对照组（n = 23）的前额叶皮层样本进行的匀浆结合分析表明，年轻自杀者中酮色林结合和减少结合的年龄依赖性差异可以用Bmax值的差异来解释。Kd值无显著差异。在我们的样本中，通过放射自显影或匀浆结合测定，性别、酒精的存在和死亡到尸检的时间不影响酮色胺的结合。
In vitro quantitative autoradiography of 5-HT2 receptors, using [3H]ketanserin as a ligand, was performed on 24 human brains postmortem. Twelve brains were donated by suicide victims and 12 by matched controls. We found a characteristic decline in 5-HT2 receptors with age in several brain regions of the control group. This age dependence of ketanserin binding was not present in some of thse brain regions of the suicide group. We also found a significant but anatomically selective reduction in the density of ketanserin binding sites in the young suicide group, compared to age-matched controls. This reduction was evident in portions of the prefrontal cortex. Homogenate binding assays on prefrontal cortex samples from a large group of suicides (n = 20) and controls (n = 23) showed that the difference in age dependence of ketanserin binding and the reduced binding in the young suicide group were explained by differences in Bmax values. No differences were observed in Kd. Sex, presence of alcohol and time from death to autopsy did not affect ketanserin binding, in our sample, as measured by either autoradiography or homogenate binding assay.