IDENTIFICATION OF A NEW BRASSINOSTEROID, CATHASTERONE, IN CULTURED-CELLS OF CATHARANTHUS-ROSEUS AS A BIOSYNTHETIC PRECURSOR OF TEASTERONE
IDENTIFICATION OF A NEW BRASSINOSTEROID, CATHASTERONE, IN CULTURED-CELLS OF CATHARANTHUS-ROSEUS AS A BIOSYNTHETIC PRECURSOR OF TEASTERONE
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DOI：
10.1271/bbb.59.1543
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发表时间：
1995-08-01
影响因子：
1.6
通讯作者：
SAKURAI, A
中科院分区：
文献类型：
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作者：
FUJIOKA, S;INOUE, T;SAKURAI, A
通过GC-MS分析长春花培养细胞中发现了一种新的油菜素类固醇(22S,24R)-3β,22-二羟基-5α-麦角斯坦-6-酮，命名为卡萨甾酮，其内源水平在2-4ng/g fw范围内，与油菜素内酯和卡斯特酮相似，使用氘标记底物的饲喂实验表明：卡萨甾酮被转化为茶甾酮和斑甾醇，这是卡萨甾酮作为油菜素类固醇自然存在的第一份报告，是茶甾酮的生物合成前体。
The occurrence of a new brassinosteroid of (22S,24R)-3 beta,22-dihydroxy-5 alpha-ergostan-6-one, named cathasterone, was demonstrated by a GC-MS analysis in cultured cells of Catharanthus roseus, Its endogenous level was in the range of 2-4ng/g fw, similar to those of brassinolide and castasterone, A feeding experiment with a deuterium-labeled substrate revealed that cathasterone was converted to teasterone and typhasterol, This is the first report of the natural occurrence of cathasterone as a brassinosteroid being the biosynthetic precursor of teasterone.